We narrowed to 21,775 results for: Sis

1. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Use

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYDE009

   Plasmid

   #194151

   Purpose

   Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   tetR-tetP-dcas9-rrnB T1-T7Te
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetp

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC-HYG-YR

   Plasmid

   #61765

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Hygromycin
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ad-Flag-XBP1s

   Plasmid

   #63678

   Purpose

   Adenoviral vector for XBP1s expression

   Depositor

   Qingbo Xu, Lingfang Zeng

   Article

   Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

   Insert

   XBP1 (XBP1 Human)
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   spliced

   Promoter

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC-CRISPR-P2A-BlastR

   Plasmid

   #98147

   Purpose

   Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis expression

   Tags

   P2A-BlastR fusion with Cas9-Nlux

   Expression

   Mutation

   Promoter

   Ribi promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. L309-myc-seipin-WT

   Plasmid

   #64061

   Purpose

   myc-tagged murine seipin (wild type); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO HIS DNAJB1

   Plasmid

   #19549

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO V5 DNAJB1

   Plasmid

   #19522

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-CRY2-mCherry-MYPT169

    Plasmid

    #178526

    Purpose

    Expresses CRY2-mCherry-MYPT (1-169 aa) in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    CRY2-mCherry-MYPT169
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ad-Flag-XBP1u

    Plasmid

    #63677

    Purpose

    Adenoviral vector for XBP1u expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)/GUG-nLuc-3XFLAG-PEST

    Plasmid

    #127314

    Purpose

    Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST_DSUP_RAMVA (pBS0353)

    Plasmid

    #185189

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD_Ub11TCA

    Plasmid

    #174517

    Purpose

    Recoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Insert

    Ubiquitin
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Recoded with TCA replacing codon 11 of wt ubiquit…

    Promoter

    araBAD

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid

    #53225

    Purpose

    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

    Depositor

    Jesse Rinehart

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Phosphoprotein synthesis

    Tags

    HIS and MBP

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli, both codons for Ser21…

    Promoter

    pLtetO

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_eA3A-nCas9

    Plasmid

    #163535

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-Tet-GBP1-C589A

    Plasmid

    #138090

    Purpose

    Lentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)

    Depositor

    Eva Frickel

    Article

    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

    Insert

    human GBP1 (GBP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    cysteine 589 to alanine (C589A)

    Promoter

    minimal CMV promoter with heptamerized tet‐operat…

    Available since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabePuro-DNAJA4CDS

    Plasmid

    #43799

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

    Insert

    DNAJA4 CDS (DNAJA4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 8, 2013

    Availability

    Academic Institutions and Nonprofits only

20. Ad-EFNA1

    Plasmid

    #102724

    Purpose

    Expression of EphrinA1

    Depositor

    Jin Chen

    Article

    Brantley-Sieders et al Cancer Res. 2006 Nov 1;66(21):10315-24.

    Insert

    EFNA1 (EFNA1 Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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