We narrowed to 14,014 results for: TIM

1. pRec

   Plasmid

   #104572

   Purpose

   Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR and LacI constitutively.

   Depositor

   Harris Wang

   Article

   Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

   Insert

   PTet-cas12, tetR, lacI
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T-3-6AOptParkin

   Plasmid

   #165107

   Purpose

   bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene

   Depositor

   Arthur Haas

   Insert

   Parkin isoform 1 (PRKN Human)
   

   Tags

   GST-6-alanine

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-51b-HaloTag11

   Plasmid

   #175519

   Purpose

   Expression of HaloTag11 in bacterial cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag11
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEB2-2ndVal mCherry2-L

   Plasmid

   #104027

   Purpose

   Plasmid encoding 2ndVal mCherry2-L

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mCherry2-L
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   mCherry2-L with valine inserted in 2nd position

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. SJZ7-KAN

   Plasmid

   #228260

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   kan
   

   Expression

   Yeast

   Promoter

   GPM1 S.c.

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SJZ4-NAT

   Plasmid

   #228257

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   nat1
   

   Expression

   Yeast

   Promoter

   FBA1 S.c.

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB1-Sapphire

   Plasmid

   #103976

   Purpose

   Plasmid encoding Sapphire

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Sapphire
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (S72A, Y145F,…

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB2-2ndVal mKate2

   Plasmid

   #104030

   Purpose

   Plasmid encoding 2ndVal mKate2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mKate2
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   mKate2 with valine inserted in 2nd position

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

   Plasmid

   #130510

   Purpose

   expresses mScarlet1

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

   Insert

   mScarlet1
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFH47

    Plasmid

    #125812

    Purpose

    Level0 LbCas12a, human codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    LbCas12a, human codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDS1902

    Plasmid

    #167278

    Purpose

    Expression plasmid for Candida albicans containing a red-shifted firefly luciferase

    Depositor

    Dominique Sanglard

    Article

    Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

    Insert

    red shifted luciferase
    

    Promoter

    ACT1

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEB2-2ndVal Katushka

    Plasmid

    #104029

    Purpose

    Plasmid encoding 2ndVal Katushka

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    2ndVal Katushka
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Katushka with valine inserted in 2nd position

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xmEGFP

    Plasmid

    #127801

    Purpose

    Homo-tetramer mEGFP-mEGFP-mEGFP-mEGFP expression vector

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    mEGFP-mEGFP-mEGFP-mEGFP
    

    Expression

    Mammalian

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. TroponinT-Gateway

    Plasmid

    #46026

    Depositor

    John Gearhart

    Article

    Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5

    Promoter

    TNNT2

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEB1-mEYFP

    Plasmid

    #103985

    Purpose

    Plasmid encoding mEYFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEYFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65G, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-VSV-G-HiBiT

    Plasmid

    #162594

    Purpose

    C-terminally HiBiT-tagged VSV-G under CMV promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    VSV-G
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEB2-TurboRFP

    Plasmid

    #103996

    Purpose

    Plasmid encoding TurboRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TurboRFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, T68A, L79F, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

    Plasmid

    #169326

    Purpose

    Expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFH13

    Plasmid

    #125798

    Purpose

    Level0 SpCas9, wheat codon optimized

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    SpCas9, wheat codon optimized
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYES2.1-pomMet1

    Plasmid

    #107444

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    Met1
    

    Tags

    V5-His

    Expression

    Yeast

    Promoter

    GAL1

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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