We narrowed to 6,931 results for: crispr cas9 plasmids

1. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFC334

   Plasmid

   #87846

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   argB
   yA specific sgRNA
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   A. nidulans gpdA promoter with Hammerhead ribozym…

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CMV-dSa-VPR mini.-syn pA

   Plasmid

   #99689

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CMV-dSa-VPR mini.-2X snRP1

   Plasmid

   #99688

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDIV489

   Plasmid

   #177697

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIV490

   Plasmid

   #177698

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CMV-dSa-VPR mini.-1X snRP1

   Plasmid

   #99687

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDIV492

   Plasmid

   #177700

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDIV491

   Plasmid

   #177699

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDIV488

    Plasmid

    #177696

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPomyces-2BD

    Plasmid

    #234428

    Purpose

    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template

    Depositor

    Min-Kyu Oh

    Article

    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

    Insert

    Modified Cas9 with polyaspartate linking
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Linked polyaspartate using glycine-serine linker

    Promoter

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX458_SSRP1_1

    Plasmid

    #72371

    Purpose

    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    SSRP1 (SSRP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PX458_SSRP1_2

    Plasmid

    #72372

    Purpose

    Encodes gRNA for 3' target of human SSRP1 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    SSRP1 (SSRP1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PX458_PBX2_1

    Plasmid

    #72357

    Purpose

    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    PBX2 (PBX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PX458_PBX2_2

    Plasmid

    #72358

    Purpose

    Encodes gRNA for 3' target of human PBX2 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Insert

    PBX2 (PBX2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid

    #68405

    Purpose

    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

    Depositor

    Jannik Elverløv-Jakobsen

    Inserts

    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    

    Use

    CRISPR

    Tags

    mTurquoise2 (BFP)

    Expression

    Mammalian

    Mutation

    Promoter

    Synthetic Probasin ARRx2 and U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML104

    Plasmid

    #67638

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML107

    Plasmid

    #67639

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation

    Depositor

    John Wyrick

    Article

    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

    Inserts

    Cas9
    single guide RNA expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pSNR52 and pTDH3 (aka GAP promoter)

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEG302 22aa SunTag NtDRMcd (noNLS) nog

    Plasmid

    #119554

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_noNLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge