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Plasmid#129402Purpose(mNeonGreen)4-tDeg fluorogenic proteinDepositorInsert(mNeonGreen)-tDeg
UseTagsExpressionMammalianMutationPromoterminiCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rictor_2 shRNA
Plasmid#1854DepositorInsertRictor (RICTOR Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro-humanU6-shRNA FASN
Plasmid#82327Purposeused to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)DepositorInsertshRNA to target Fatty Acid Synthase (FASN) (FASN Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRTagsExpressionMammalianMutationPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
U6.3>Control.gRNA.f+e
Plasmid#99140PurposeControl gRNA under the regulation of an optimized chicken specific U6 promoterDepositorInsertControl gRNA
UseCRISPRTagsExpressionMutationPromoterChicken U6.3Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos6_AmpR
Plasmid#119149PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZO-1 shRNA 3253
Plasmid#37206DepositorInsertdog ZO-1 shRNA (TJP1 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
ZO-1 shRNA 2968
Plasmid#37208DepositorInsertdog ZO-1 shRNA (TJP1 canine)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pICSL11015
Plasmid#117499PurposeFAST-Red selectable marker Golden Gate Level 1 Position 1DepositorInsertBpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
UseTagsRFPExpressionPlantMutationPromoterAtOLE1Available sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
gRNA-POLQ-Halo
Plasmid#211636PurposesgRNA against POLQDepositorInsertsgRNA POLQ
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458_CEBPB_2
Plasmid#64047PurposeExpresses gRNA against human CEBPB along with Cas9 with 2A GFPDepositorInsertgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR BFP-guide1
Plasmid#118157PurposeCRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the BFP CDSDepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GESTALT_pX330-v1
Plasmid#103061Purposeplasmid px330 with guide targeting GESTALT v1 through V5 constructsDepositorInsertintegration of Cas9 with guide targeting GESTALT barcodes V1 to V5
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJF131B
Plasmid#113326PurposepTet-W108 scRNA.b2, pTet-RR2-sgRNADepositorInsertW108 scRNA_MS2 and sgRNA_RR2
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-AcrB/AcrD/AcrFa, ABO_0964
Plasmid#86206PurposeExpresses efflux pump in E. coli to provide resistance to biofuel (low copy)DepositorInsertEfflux pump
UseTagsExpressionBacterialMutationPromoterATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…Available sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-U6-RNF103-CHMP3
Plasmid#140584PurposeExpresses a gRNA and mCherry in mammalian cellsDepositorInsertgRNA RNF103-CHMP3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Crispr-V2-HMID.v1
Plasmid#103062PurposeLentiCrisprV2 with guide targeting V1-V5 GESTALT barcodesDepositorInsertCas9 plus expressed GESTALT V1-V5 guide
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos7_AmpR
Plasmid#119150PurposeEncodes sgRNA targeting position 7 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 7 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos5_AmpR
Plasmid#119148PurposeEncodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 5 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only