We narrowed to 3,243 results for: phage

1. pJT173_GalL_A3A-R128A-BE3

   Plasmid

   #145122

   Purpose

   Expressing base editor A3A-R128A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(R128A)-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(R128A); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

   Plasmid

   #133795

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDsRed-attP

   Plasmid

   #51019

   Purpose

   Vector for generating dsDNA donors for homology-directed repair to replace genes or other genomic sequence with an attP docking site. Contains the visible marker 3xP3-DsRed. As known as pHD-DsRed-attP

   Depositor

   Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Inserts

   attP
   LoxP-3xP3-DsRed-LoxP
   

   Use

   High copy, amp resistance

   Promoter

   3xP3 and None

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b_H6-Smt3-AesC-GFP

   Plasmid

   #234486

   Purpose

   Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   C-terminal Fragment of the Aes123 PolB1 Split Intein
   

   Tags

   Hexahistidine Tag, Superfolder Green Fluorescent …

   Expression

   Bacterial

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL-c2X_MBP-CLmN-H6

   Plasmid

   #234487

   Purpose

   Maltose binding protein N-terminally fused to His-tagged CLmN split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   N-terminal Fragment of the CLm Split Intein
   

   Tags

   Hexahistidine Tag and Maltose Binding Protein (MB…

   Expression

   Bacterial

   Mutation

   T69K, F75H, M118N within the N-terminal fragment …

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSara

   Plasmid

   #60720

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   N Terminal fragment of T7 RNAP split betwen 179-180
   N Terminal fragment of T7 RNAP split betwen 179-180
   

   Expression

   Bacterial

   Mutation

   T7 RNAP split between positions 179-180 and wt T7…

   Promoter

   PBAD

   Available Since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPT234

   Plasmid

   #127855

   Purpose

   Repressilator 2.0, without degradation tags, integrated triple reporter

   Depositor

   Johan Paulsson

   Article

   Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

   Inserts

   bla
   mSCFP3
   mKate2
   mVenus
   TetR
   LacI
   cI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   First 11aa derived from mCherry as fusion protein

   Promoter

   pAmpR, pL LacO1, pL tetO1, and pR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTSlb-N748D

    Plasmid

    #60732

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEVOL-AcKRS-CloDF

    Plasmid

    #127415

    Purpose

    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

    Plasmid

    #160633

    Purpose

    TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31-RDF
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTSlb-Q758C

    Plasmid

    #60729

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations Q758C

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8e(TadA-8e K20A R21A)

    Plasmid

    #138496

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e K20A R21A)-nSpCas9
    

    Expression

    Mammalian

    Mutation

    TadA-8e K20A R21A

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 PhiC31 attP:TMtb:P35S:attB (GB1494)

    Plasmid

    #160577

    Purpose

    35S promoter sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB)

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31 PB (attP:TMtb:P35S:attB)
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTSara-R756K

    Plasmid

    #60727

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTSlb-R756K

    Plasmid

    #60731

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations R756S

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTSara-N748D

    Plasmid

    #60726

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAX1_HS∆tum

    Plasmid

    #225198

    Purpose

    Used to create a markerless deletion of the E. coli HS tum gene

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    tum homology arms
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2h70CRET(#325)

    Plasmid

    #184060

    Purpose

    cyclofen-inducible CRE activation in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

    Insert

    CRE-ERT2
    

    Use

    Zebrafish transient transgenesis

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Hsp70-4

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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