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CLK1 gRNA (BRDN0001147408)

Plasmid

#76233

Purpose

3rd generation lentiviral gRNA plasmid targeting human CLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CLK1 (CLK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-VQR-GFP_activity_reporter

Plasmid

#87156

Purpose

This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted).

Depositor

Daniel Bauer

Article

Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

Insert

GFP and sgRNA targeting GFP (NGA restricted)

Use

Lentiviral

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PIK3CA gRNA (BRDN0001148028)

Plasmid

#77418

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-BAP1-G312G-HA

Plasmid

#154022

Purpose

Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

c.936T>G, p.G312G (Synonymous Mutation) by Sit…

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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PIK3CA gRNA (BRDN0001145530)

Plasmid

#77416

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CA (PIK3CA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ELMOD3

Plasmid

#155584

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ELMOD3 (ELMOD3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DMGDH

Plasmid

#155549

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DMGDH (DMGDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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DHX40

Plasmid

#155540

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DHX40 (DHX40 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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DDX56

Plasmid

#155530

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX56 (DDX56 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CIR1

Plasmid

#155452

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CIR1 (CIR1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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