Addgene

We narrowed to 3,518 results for: mak

Showing: 1721 - 1740 of 3518 results

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pHYBAD94

Plasmid

#42305

Depositor

Hideo Iwai

Article

Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

Insert

NpuDnaEC6

Use

Tags

GB1-H6

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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human pirh2-(138-261)

Plasmid

#24882

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains aa 138-261.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
human pirh2-(187-261)

Plasmid

#24883

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Contains aa 187-261.

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pEF.DEST51-mScarlet

Plasmid

#207960

Purpose

Vector for overexpression of the mScarlet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Use

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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FH-TET3-pEF

Plasmid

#49446

Purpose

This plasmid expresses full-length human TET3.

Depositor

Anjana Rao

Article

Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

Insert

Tet methylcytosine dioxygenase 3 (TET3 Human)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

EF-1alpha

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-WT-Highest-CAR_RHL004

Plasmid

#164826

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspWYL1

Plasmid

#108306

Purpose

Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-High-CAR_RHL002

Plasmid

#164825

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-High CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-High Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE PGK-GFP-IRES-LUC-W

Plasmid

#46793

Purpose

2nd generation lentiviral vector

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Inserts

PGK
eGFP (eGFP )
IRES
Luciferase

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4KB

Plasmid

#221759

Purpose

Stable expression of GFP-PI4KB in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4KB (PI4KB Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-C(3+)-EiP

Plasmid

#21313

Purpose

Lentiviral EOS reporter with Oct3/4 enhancer (x3), expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-C(3+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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PL-SIN-EOS-S(4+)-EiP

Plasmid

#21314

Purpose

Lentiviral EOS reporter with Sox2 enhancer (x4), expresses EGFP and Puro resistance

Depositor

James Ellis

Article

Hotta et al Nat Methods. 2009 May . 6(5):370-6.

Insert

Enhanced Green Fluorescence Protein, Puromycin resistance gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EOS-S(4+) promoter region

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pHAGE-CMV-hAAT-W

Plasmid

#126704

Purpose

Constituitive, ubiquitious human alpha - 1 antitrypsin gene expression

Depositor

Darrell Kotton

Article

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

hAAT (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMVp

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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