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pET28b-Sad1

Plasmid

#90297

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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p414TEF_Hsp82_CycTER

Plasmid

#61711

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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U6-U57C

Plasmid

#169928

Purpose

WT U6 with a U57C mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57C

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pWZ414-F53

Plasmid

#23077

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F30

Plasmid

#23066

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 9 to Glutamine H3 K9Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F31

Plasmid

#23067

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 changed lysine 14 to Glutamine K14Q

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pET28b-sad1-C63A

Plasmid

#90302

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Mutation

C63A

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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YEplac181-GAL11

Plasmid

#8628

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

GAL11 (GAL11 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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Gal-Rad51 KR

Plasmid

#33232

Depositor

Hannah Klein

Article

Shah et al Mol Cell. 2010 Sep 24;39(6):862-72.

Insert

Rad51 (RAD51 Budding Yeast)

Expression

Yeast

Mutation

K191R

Promoter

Gal1

Available Since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

K351N

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pFC663

Plasmid

#27324

Depositor

Fred Cross

Article

Cross et al Genetics. 2007 Jul . 176(3):1541-55.

Insert

SIC1 (SIC1 Budding Yeast)

Expression

Yeast

Mutation

Contains insertion relative to SGDB sequence of G…

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pGALSc104(G217S/T499I)

Plasmid

#1151

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

G217S/T499I

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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GAL-CDC12(E368Q)

Plasmid

#1164

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Expression

Yeast

Mutation

E368Q

Promoter

GAL

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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p414GPD_Hsp82_CycTER

Plasmid

#61710

Purpose

Yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

GPD

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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TM104b(A503V/A509D)

Plasmid

#1161

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

A503V/A509D

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
TM104b(G217S/T499I)

Plasmid

#1160

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Expression

Yeast

Mutation

G217S/T499I

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pFUW mTagBFP2-P2A-NeoR

Plasmid

#239568

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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