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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,308 results for: sequence

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Showing: 8701 - 8710 of 13308 results

  1. pCW57-CMV-MAAP-SL08V9

    Plasmid
    #218251
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218251-plasmid-map-sequence-id-449683

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  2. px552-sg-gria1-HT

    Plasmid
    #187652
    Purpose
    Contains HaloTag to be inserted into the NTD of Gria1 (via HITI), single guide RNA to target Cas9 to Gria1 under control of the U6 promoter, and miRFP670 under control of human synapsin promoter.
    Depositor
    Erin O'Shea
    Article
    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75
    Inserts
    HaloTag donor sequence
    miRFP670
    Use
    AAV and CRISPR
    Tags
    N/A
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    187652-plasmid-map-sequence-id-369120

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  4. pCW57-CMV-MAAP9

    Plasmid
    #218246
    Purpose
    1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218246-plasmid-map-sequence-id-449800

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  7. pMAT_M13_6xSAG

    Plasmid
    #197096
    Purpose
    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C
    Depositor
    Wendell Lim
    Article
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    Expression
    Mammalian
    Available Since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197096-plasmid-map-sequence-id-386824

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  8. pET23a-H6-TEV-AncSZ

    Plasmid
    #214235
    Purpose
    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation
    Depositor
    Neel Shah
    Article
    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:
    Insert
    Syk kinase (SYK Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence gained through ancestral reconstruction …
    Promoter
    T7
    Available Since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214235-plasmid-map-sequence-id-422817

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  9. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid
    #213401
    Purpose
    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    Expression
    Plant
    Promoter
    2x35S
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213401-plasmid-map-sequence-id-422351

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  10. pSC-cUnaG-LPETG

    Plasmid
    #207635
    Purpose
    A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.
    Depositor
    Cole DeForest
    Article
    Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1
    Insert
    photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Amber stop codon at SC's critical lysine
    Promoter
    T7
    Available Since
    Oct. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    207635-plasmid-map-sequence-id-409865

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Showing: 8701 - 8710 of 13308 results