We narrowed to 2,382 results for: T2A

1. Multiplex-CRISPR-miR-200FAM-T2A-GFP

   Plasmid

   #179856

   Purpose

   to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b

   Depositor

   Brendan Jenkins

   Article

   Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413

   Insert

   hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

   Plasmid

   #195503

   Purpose

   SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
   

   Use

   CRISPR; Donor vector

   Tags

   T2A-H2B-mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti spCas9 T2A TagRFP-T P2A puro

   Plasmid

   #122200

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9
   TagRFP-T
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

   Plasmid

   #191676

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

   Depositor

   Veerle Baekelandt

   Inserts

   ATP10B (ATP10B Human)
   TMEM30A (TMEM30A Human)
   

   Use

   Lentiviral

   Tags

   6x HIS and flag

   Expression

   Mutation

   D433N

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEpicVector3mC_Puro-T2A-mCherry-ProtC-FLAG-AU1

   Plasmid

   #162117

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to ProtC-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   ProtC-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEpicVector3mC_Puro-T2A-mCherry-VSVg-FLAG-AU1

   Plasmid

   #162108

   Purpose

   mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; mCherry linked to VSVg-FLAG-AU1
   

   Use

   Lentiviral

   Tags

   VSVg-FLAG-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEpicVector3G_Puro-T2A-EGFP-VSVg-ProtC-FLAG

   Plasmid

   #162084

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to VSVg-ProtC-FLAG
   

   Use

   Lentiviral

   Tags

   VSVg-ProtC-FLAG

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-AU1

   Plasmid

   #162091

   Purpose

   EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

   Depositor

   Julien Sage

   Article

   Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

   Insert

   Puromycin resistant; EGFP linked to StrepTagII-ProtC-AU1
   

   Use

   Lentiviral

   Tags

   StrepTagII-ProtC-AU1

   Expression

   Mutation

   WT

   Promoter

   EF1A

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-ef1a-dCasRx-FTO-HA-T2A-BSD

    Plasmid

    #177120

    Purpose

    dCasRx mediated RNA tethering of FTO

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    dCasRx-FTO
    

    Use

    Lentiviral

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    dCasRX contains R239A/H244A/R858A/H863A mutations…

    Promoter

    Ef1a

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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