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Showing: 1741 - 1760 of 2316 results

  1. pLY134

    Plasmid
    #130962
    Purpose
    Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEB5
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Anderson promoter J23100
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    130962-plasmid-map-sequence-id-257714

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  3. psecBFP2

    Plasmid
    #62230
    Purpose
    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.
    Depositor
    Erik Snapp
    Article
    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;
    Insert
    secBFP2
    Use
    Tags
    secbfp2
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. pLSLZ

    Plasmid
    #51495
    Purpose
    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    51495-plasmid-map-sequence-id-78592

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  8. PC2328

    Plasmid
    #232089
    Purpose
    Mammalian expression plasmid for deubiquibody (duAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and OTUB1 catalytic domain.
    Depositor
    Pranam Chatterjee
    Article
    Hong et al Res Sq [Preprint]. 2024 Jul 26:rs.3.rs-4
    Insert
    duAb with cloning Esp3I restriction site
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232089-plasmid-map-sequence-id-469903

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  9. pYPQAT49

    Plasmid
    #223421
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223421-plasmid-map-sequence-id-454169

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  10. pYPQAT50

    Plasmid
    #223422
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for monocot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA was driven by separate ZmUbi1 promoter; Hygromycin for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-LbCas12a-RRV-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223422-plasmid-map-sequence-id-453572

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  11. pYPQAT51

    Plasmid
    #223423
    Purpose
    T-DNA vector for LbCas12a-RRV based mutagenesis for dicot plants; highly efficient including TTV PAM; LbCas12a-RRV and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-LbCas12a-RRV-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223423-plasmid-map-sequence-id-453500

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  13. pHPHS800

    Plasmid
    #228240
    Purpose
    Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Insert
    Cas9
    Use
    Tags
    Hygromycin phosphotransferase
    Expression
    Mammalian
    Mutation
    Promoter
    none
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228240-plasmid-map-sequence-id-454866

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  14. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Use
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  16. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  17. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  18. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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Showing: 1741 - 1760 of 2316 results