We narrowed to 8,022 results for: Dos;

1. nls-mCherry

   Plasmid

   #108881

   Purpose

   Expression of nuclear localized mCherry

   Depositor

   Rob Parton

   Article

   Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

   Insert

   nls-mCherry
   

   Use

   Can be used for mammalian expression or in vitro …

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-I3-01-Myc

   Plasmid

   #80038

   Purpose

   Expression of I3-01

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   I3-01-Myc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. gRNA_AAVS1-T1

   Plasmid

   #41817

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Rab9a

   Plasmid

   #78592

   Purpose

   Express mCherry-Rab9a in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   Rab9a (RAB9A Human)
   

   Use

   Tags

   mCherry2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK-flag-USP19

   Plasmid

   #78597

   Purpose

   Express flag-USP19 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-SWAP70

   Plasmid

   #84581

   Purpose

   Expresses GFP-tagged SWAP70 in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

   Insert

   SWAP70 (SWAP70 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pMpGWB300

   Plasmid

   #68628

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEDA5_GFPmut3_Y66H

   Plasmid

   #80085

   Purpose

   Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

   Depositor

   Timothy Whitehead

   Article

   Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

   Insert

   GFPmut3_Y66H
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   changed Tyrosine 66 to Histidine

   Promoter

   proB

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUB-MSP1D1

    Plasmid

    #161609

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    UD-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1 ( Q138W, L181W, Y236W)

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-LacO-LgBiT

    Plasmid

    #216779

    Purpose

    Expresses LgBiT in bacteria

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    LgBiT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOEM1_pCMV:hShh-pPH:vsvged

    Plasmid

    #111156

    Purpose

    Baculovirus rescue vector, VSVGED pseudotype, hShh

    Depositor

    Elly Tanaka

    Article

    Nacu et al Nature. 2016 May 19;533(7603):407-10. do

    Insert

    hShh (SHH Human)
    

    Use

    Baculoviral rescue shuttle vector

    Tags

    Expression

    Insect and Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTEF6-7

    Plasmid

    #124583

    Purpose

    Reporter plasmid with constitutively expressed mCherry and YFP (eCitrine)

    Depositor

    Matthias Heinemann

    Article

    Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sirius-mT-Sapphire_pcDNA3

    Plasmid

    #89584

    Purpose

    Genetically encoded ratiometric fluorescent thermometer

    Depositor

    Takeharu Nagai

    Article

    Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

    Insert

    Sirius-mT-Sapphire
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME phluorin2-gpi

    Plasmid

    #73793

    Purpose

    ratiometric pHluorin2 with a gpi linkage sequence in a middle entry clone

    Depositor

    David Raible

    Article

    Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

    Insert

    ratiometric pHluorin2-gpi
    

    Use

    Middle entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD-MSP1D1

    Plasmid

    #161607

    Purpose

    Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

    Depositor

    Stephen Sligar

    Article

    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

    Insert

    D-MSP1D1
    

    Use

    Tags

    7-His tag

    Expression

    Bacterial

    Mutation

    mutant of MSP1D1; all tryptophans were replaced b…

    Promoter

    T7

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N1-hAIM2

    Plasmid

    #51542

    Purpose

    Express GFP-tagged hAIM2 in mammalian cells

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB201

    Plasmid

    #68592

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMpGWB129

    Plasmid

    #68583

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    tdTomato

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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