We narrowed to 1,186 results for: MS2;

1. Cas12f-GE ver4.0

   Plasmid

   #176543

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.0
   Cas12f-GE ver4.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPLINTR GFP v1 backbone

   Plasmid

   #179777

   Purpose

   SPLINTR backbone vector

   Depositor

   Mark Dawson

   Article

   Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas12f-GE ver3.0

   Plasmid

   #176508

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver3.0
   Cas12f-GE ver3.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

   Plasmid

   #160571

   Purpose

   Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNAscaffold 2.1 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. (attB) tdMCP-tagged BRaf

   Plasmid

   #226165

   Purpose

   Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-BRaf (BRAF Human)
   

   Use

   Bxb1 attb, no promoter

   Tags

   3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2 sgRNA sCRNA 2.0 (GB2461)

   Plasmid

   #160593

   Purpose

   Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA sCRNA 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ269E2

   Plasmid

   #164721

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Lesley Forrester, Antonella Fidanza

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

   Plasmid

   #160568

   Purpose

   tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_1-pTRNA-scf 2.1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid

    #160621

    Purpose

    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    U6-26:gRNA 4 (pNos): 2.1 aptamer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    U6-26

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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