Addgene

We narrowed to 5,877 results for: pcDNA 3.1

Showing: 2641 - 2670 of 5877 results

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pcDNA3 Rod Opsin

Plasmid

#109361

Purpose

Human rod opsin

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin (RHO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pZ149-pcDNA3-BhCas12b_v4

Plasmid

#122446

Purpose

Expresses BhCas12b_v4 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BhCas12b_v4-NLS-3xHA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRA-polyA

Plasmid

#59781

Purpose

Express H3.3 chaperone HIRA in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA-Golgi-ZapCY1

Plasmid

#36322

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

Golgi-ZapCY1 high affinity Zn(II) sensor

Use

Tags

GalT (first 60 amino acids)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pZ143-pcDNA3-BvCas12b

Plasmid

#122445

Purpose

Expresses BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

CMV-NLS-BvCas12b-NLS-3xHA

Use

Tags

3XHA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-YOD1

Plasmid

#49784

Purpose

Express YOD1 with a N-terminal Flag-tag in mammalian cells

Depositor

Christian Schlieker

Article

Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

Insert

YOD1 (YOD1 Human)

Use

Tags

Flag-tag

Expression

Mutation

WT

Promoter

Available since

Dec. 16, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Dub3 mouse

Plasmid

#72682

Purpose

mammalian expression of mouse Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
vector-dendra2 PCDNA

Plasmid

#121134

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

empty cassete for contol of miR expression

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HIRAmut-polyA

Plasmid

#59782

Purpose

This Hira gene contains silent mutations in siRNA-targeted sequences (see Inoue and Zhang, 2014 NSMB).

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

HIRA

Use

Tags

Expression

Mammalian

Mutation

Change from 'GATCGAAGTTTG' to 'GAc…

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S276A

Plasmid

#42924

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Use

Tags

Expression

Mammalian

Mutation

Ser276 to Ala

Promoter

CMV

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-PuRRRE

Plasmid

#208327

Purpose

C-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-PuRRRE

Use

Tags

PuRRRE

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-TREER

Plasmid

#208325

Purpose

C-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).

Depositor

Kimberly Beatty

Article

Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

Insert

TfR1-TREER

Use

Tags

TREER

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A

Plasmid

#42917

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Use

Tags

Expression

Mammalian

Mutation

Ser110 to Ala

Promoter

CMV

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S298ter

Plasmid

#42925

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Use

Tags

Expression

Mammalian

Mutation

Ser298 to stop codon

Promoter

CMV

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-GATA3-DCzf

Plasmid

#1333

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 MYC-Sumo3Q89P

Plasmid

#48963

Purpose

Expresses MYC-Sumo3Q90P mutant in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Use

Tags

MYC (EQKLISEED)

Expression

Mammalian

Mutation

Mutated Q89P

Promoter

T7

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-LOV-VP16

Plasmid

#42499

Purpose

LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

FKF1 (N-LOV, G128D mutant) fused with VP16

Use

Tags

F-type VP16 activation domain and HA tag

Expression

Mammalian

Mutation

G128D 1-168aa

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-I-PhoCl-HCVp

Plasmid

#87697

Purpose

Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)

Use

Tags

EBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-mCherry-4A/4B-CAAX

Plasmid

#87699

Purpose

Express mCherry with a peptide linker (contaning a HCVp 4A/4B substrate site) and a C-terminal CAAX box from KRras for plasma-membrane targeting in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

mCherry-4A/4B-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-KIRIN1

Plasmid

#107416

Purpose

Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL3-FLAG

Plasmid

#15820

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin3 (CUL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N418

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8.6-535

Plasmid

#87125

Purpose

Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

Insert

Renilla Luciferase

Use

Tags

Expression

Mammalian

Mutation

RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAB-ICUE

Plasmid

#118480

Purpose

ddRFP-based single-color biosensor for monitoring cAMP dynamics.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-ICUE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin4B (CUL4B Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MYC-Sumo3 WT

Plasmid

#48964

Purpose

Expresses MYC-Sumo3 Wild Type in mammalian cells

Depositor

Guy Salvesen

Article

Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

Insert

SUMO3 (SUMO3 Human)

Use

Tags

MYC (EQKLISEED)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Smac(1-60)mCherry

Plasmid

#40880

Depositor

Douglas Green

Article

Tait et al Dev Cell. 2010 May 18;18(5):802-13.

Insert

Smac(1-60)mCherry (DIABLO Human)

Use

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-human OCRL

Plasmid

#22207

Depositor

Pietro De Camilli

Article

Erdmann et al Dev Cell. 2007 Sep . 13(3):377-90.

Insert

Oculocerebrorenal syndrome of Lowe (OCRL Human)

Use

Retroviral

Tags

HA

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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