We narrowed to 15,323 results for: pET
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Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
TagsEGFPExpressionBacterialPromoterPemrRAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 [315-445]
Plasmid#186474PurposeExpression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
Tags6HISExpressionBacterialMutationMSP domain [315-445]Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b MOSPD2 RD/LD [282-490]
Plasmid#186470PurposeExpression of human MOSPD2 (MSP domain from residue 282 to 490; mutant R404D/L406D, unable to bind FFAT motifs), fused to a 6HIS tag, in bacteriaDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
Tags6HISExpressionBacterialMutationMSP domain [282-490]Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303C-hSOD1 A4V/C6S
Plasmid#139667PurposeExpression plasmids containing human A4V/C6S SOD1DepositorInsertSuperoxide dismutase-1 (SOD1 Human)
ExpressionBacterialMutationchange alanine 4 to valine, change cysteine 6 to …PromoterT7Available SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
ExpressionMammalianPromoterPhCMV / PPGK / RPBSAAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH230-2-Tier1-PmPGK1-YPet
Plasmid#169521PurposeTier-1 vector encoding PmPGK1-driven YPet expression (PmPGK1-YPet-pA).DepositorInsertPPGK-driven yellow fluorescent protein Ypet
ExpressionMammalianPromoterPPGKAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2388-Tier1-PhCMV-p2a_Ypet
Plasmid#169525PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).DepositorInsertPCMV-driven P2A-fused yellow fluorescent protein Ypet
ExpressionMammalianPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFOX8-Tier1-PhCMV-YPet
Plasmid#169506PurposeTier-1 vector encoding PhCMV-driven YPet expression (PhCMV-YPet-pA).DepositorInsertPCMV-driven yellow fluorescent protein Ypet
ExpressionMammalianPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
3020_pETcon-SARS-CoV-2-RBD_N501Y
Plasmid#184405Purposeyeast surface display of the SARS-CoV-2 Alpha variant RBDDepositorInsertSARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
TagsHA and c-MycExpressionYeastMutationN501YAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_mouse-Caspase-1-H340D_enzymatic-domain
Plasmid#183388PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1 (AA130-404)
TagsHis-TEVExpressionBacterialMutationH340DPromoterT7Available SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_canine-Caspase-1/4_enzymatic-domain
Plasmid#183383PurposeOverexpression of catalytic domain of inflammatory caspase in bacteriaDepositorInsertCaspase-1/4 (AA130-404) (CASP4 Canis lupus familiaris)
TagsHis-TEVExpressionBacterialPromoterT7Available SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmGe1_1220-1354_G
Plasmid#146420PurposeBacterial Expression of DmGe1_1220-1354DepositorInsertDmGe1_1220-1354 (Ge-1 Fly)
ExpressionBacterialAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmEDC3_331-440_H
Plasmid#146448PurposeBacterial Expression of DmEDC3_331-440DepositorInsertDmEDC3_331-440 (Edc3 Fly)
ExpressionBacterialAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-UTX(420-633)-H6
Plasmid#183773PurposeExpress pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.DepositorInsertUTX
Tags6 His tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmBelle_695-798_B
Plasmid#145961PurposeBacterial Expression of DmBelle_695-798DepositorInsertDmBelle_695-798 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_1-298_B
Plasmid#145964PurposeBacterial Expression of DmBelle_1-298DepositorInsertDmBelle_1-298 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_695-798_B
Plasmid#145965PurposeBacterial Expression of DmBelle_695-798DepositorInsertDmBelle_695-798 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_1-694_B
Plasmid#145951PurposeBacterial Expression of DmBelle_1-694DepositorInsertDmBelle_1-694 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_299-694_B
Plasmid#145952PurposeBacterial Expression of DmBelle_299-694DepositorInsertDmBelle_299-694 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_299-798_B
Plasmid#145953PurposeBacterial Expression of DmBelle_299-798DepositorInsertDmBelle_299-798 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-11-6His-DmBelle_695-798_B
Plasmid#145954PurposeBacterial Expression of DmBelle_695-798DepositorInsertDmBelle_695-798 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmBelle_1-298_B
Plasmid#145957PurposeBacterial Expression of DmBelle_1-298DepositorInsertDmBelle_1-298 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmBelle_1-694_B
Plasmid#145958PurposeBacterial Expression of DmBelle_1-694DepositorInsertDmBelle_1-694 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmBelle_299-694_B
Plasmid#145959PurposeBacterial Expression of DmBelle_299-694DepositorInsertDmBelle_299-694 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmBelle_299-798_B
Plasmid#145960PurposeBacterial Expression of DmBelle_299-798DepositorInsertDmBelle_299-798 (bel Fly)
ExpressionBacterialAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-DmGW182_1-539_F
Plasmid#146264PurposeBacterial Expression of DmGW182_1-539DepositorInsertDmGW182_1-539 (gw Fly)
ExpressionBacterialMutationone non silent mutation G349A (G117R) compared to…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E60amb)-His
Plasmid#168043PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationGlu 60 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-DogTag Loop C
Plasmid#171781PurposeExpresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-DogTag Loop C
TagsHis6ExpressionBacterialMutationDogTag and linkers inserted between residues Asp1…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop B
Plasmid#171777PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103DepositorInsertsfGFP-SpyTag003 Loop B
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP-SpyTag003 Loop C
Plasmid#171778PurposeExpresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174DepositorInsertsfGFP-SpyTag003 Loop C
TagsHis6ExpressionBacterialMutationSpyTag003 and linkers inserted between residues A…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (E143amb)-His
Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b p97-His D592Amb
Plasmid#169022PurposeExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteriaDepositorInsertp97
TagsHisExpressionBacterialMutationD592TAGPromoterT7Available SinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
Tags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable SinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETCON-Aga2p-TAPBPR-myc
Plasmid#153486PurposeYeast expression of Aga2p-TAPBPR-mycDepositorAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
ExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-DAD2 cPGFP(A149)
Plasmid#165998PurposeExpression of coding sequence in Escherichia coliDepositorInsertDAD2 cPGFP(A149)
ExpressionBacterialPromoterT7 promoterAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GVcl 1-1066 /*TBM
Plasmid#162781Purposeamplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain IIDepositorArticleAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only