We narrowed to 17,109 results for: form
-
Plasmid#228083PurposeMammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers, N-term TagBFP, and C-term P6f; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2h, S4h, P6f
ExpressionMammalianAvailable SinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Q54A
Plasmid#217912PurposeA plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutationDepositorInsertPXP Q54A
Tags6x His (N and C term)ExpressionBacterialMutationGlutamine at position 55 and 267 mutated to Alani…PromoterT5Available SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
T40A
Plasmid#217911PurposeA plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutationDepositorInsertPXP T40A
Tags6x His (N and C term)ExpressionBacterialMutationThreonine at position 41 and 253 mutated to Alani…PromoterT5Available SinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA265
Plasmid#215967PurposeFragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoterDepositorHas ServiceCloning Grade DNAInsertSFFV_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUASt-Xbp1-HA-GFP-3'UTR Spl
Plasmid#201246PurposeXbp1 tagged with HA and GFP followed by 3'UTR of xbp1spliced formDepositorInsertdXbp1 (Xbp1 Fly)
ExpressionInsectAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
Plasmid#191631PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase (R506S mutation, inactive form)
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xMS2 no wild type
Plasmid#158204PurposeCassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.DepositorInsert10 binding sites of MS2 without the WT sequence from oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xQb no wild type
Plasmid#158205PurposeCassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.DepositorInsert10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX-FLAG-CH3 Del33
Plasmid#133724PurposeRetroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlagMutationpartial (not complete) p300 fragment, AT and CH3 …PromoterCMVAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLB(N)CX AT-CH3-FLAG-HA WY
Plasmid#133725PurposeRetroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).DepositorInsertEP300 (EP300 Human)
UseRetroviralTagsFlag and HAMutationpartial (not complete) p300 fragment, AT and CH3 …Available SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Hemmar-DSCP-ftz-FLP(D)
Plasmid#119894PurposeFLP(D) with a Drosophila Synthetic Core Promoter (DSCP) and ftz intronDepositorInsertsUseDrosophila expressionMutationa mutated form of FLP, which at position 5 contai…Available SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R47A)
Plasmid#112722PurposeArg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R47A)
Tags6His-TEVExpressionBacterialMutationR47APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)Y815A
Plasmid#100744PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutantDepositorAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio
Plasmid#80325PurposeExpresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
TagsBiotinExpressionMammalianMutationH170A and H256APromoterCMVAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NET39 Short pmRFP-N2 (451)
Plasmid#61981Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET39 (PPAPDC3 Human)
TagsmRFPExpressionMammalianMutationShort form of NET39PromoterCMVAvailable SinceAug. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorAvailable SinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-polyQ74-NLS
Plasmid#231892PurposeForms nuclear HTT partial exon 1 Q74-NLS aggregatesDepositorInsertHTT partial exon 1 Q74-NLS (HTT Human)
TagsEGFP and SV40 NLSExpressionMammalianMutationCAG repeat expansionAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 hSCN5A-L325R
Plasmid#145373Purposeexpresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cellsDepositorInsertsodium voltage-gated channel alpha subunit 5 (SCN5A Human)
ExpressionMammalianMutationchanged Leucine 325 to ArgininePromoterCMVAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only