Addgene

We narrowed to 903 results for: TRP1

Showing: 401 - 450 of 903 results

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pSP566

Plasmid

#139500

Purpose

Gene tagging vector to add mCherry Tag and membrane anchoring motif

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

mCherry-CaaX

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBTMcC24-DM

Plasmid

#111236

Purpose

yeast expression vector

Depositor

Ulrich Stelzl

Article

Woodsmith et al Nat Methods. 2017 Dec;14(12):1213-1221.

Insert

ccdB-cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRS414-ATG41

Plasmid

#113080

Purpose

Contain 1,000 nucleotides of the ATG41 5'UTR, the ATG41 open reading frame and 500 nucleotides of the 3' UTR.

Depositor

Daniel Klionsky

Article

Yao et al Autophagy. 2015;11(12):2288-99. doi: 10.

Insert

ATG41

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

ATG41 endogenous promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314 snu114-50

Plasmid

#111315

Purpose

pRS314 snu114-50

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-50 (C928R)

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-del(IV)

Plasmid

#111321

Purpose

snu114-del(IV)

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

deletion of domain IVa

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-15

Plasmid

#111312

Purpose

pRS314 snu114-15

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-15 (K146I)

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-14

Plasmid

#111311

Purpose

snu114-14

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-14

Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314 snu114-40

Plasmid

#111314

Purpose

snu114-40

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-40 (M842R)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-30

Plasmid

#111313

Purpose

snu114-30

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-30 (G646R)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114-60

Plasmid

#111316

Purpose

pRS314 snu114-60

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114-60 (K939stop)

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS314 snu114(G145A)

Plasmid

#111319

Purpose

snu114(G145A)

Depositor

Christine Guthrie

Article

Brenner et al Genetics. 2005 Jul;170(3):1063-80. doi:

Insert

SNU114

Use

Tags

Expression

Yeast

Mutation

snu114 G145A

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pVEG16*

Plasmid

#111855

Purpose

Xylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).

Depositor

Nikhil Nair

Article

Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

Insert

GAL3p-GAL3-Syn4.1-TEFt

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

D68N, V69M, A109V, I271L

Promoter

GAL3

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Hsh155 103-972

Plasmid

#111989

Purpose

Truncated Hsh155 (aa 103-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 103-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

103-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 96-972

Plasmid

#111988

Purpose

Truncated Hsh155 (aa 95-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 96-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

96-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 237-972

Plasmid

#111992

Purpose

Truncated Hsh155 (aa 237-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 237-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

237-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 64-972

Plasmid

#111987

Purpose

Truncated Hsh155 (aa 64-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 64-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

64-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-956

Plasmid

#111999

Purpose

Truncated Hsh155 (aa 1-956)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-956

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-956 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-951

Plasmid

#112001

Purpose

Truncated Hsh155 (aa 1-951)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-951

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-951 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-966

Plasmid

#111997

Purpose

Truncated Hsh155 (aa 1-966)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-966

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-966 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 472-972

Plasmid

#111996

Purpose

Truncated Hsh155 (aa 472-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 472-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

472-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 351-972

Plasmid

#111995

Purpose

Truncated Hsh155 (aa 351-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 351-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

351-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 310-972

Plasmid

#111994

Purpose

Truncated Hsh155 (aa 310-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 310-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

310-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 275-972

Plasmid

#111993

Purpose

Truncated Hsh155 (aa 275-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 275-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

275-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 157-972

Plasmid

#111990

Purpose

Truncated Hsh155 (aa 157-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 157-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

157-972 truncation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-961

Plasmid

#111998

Purpose

Truncated Hsh155 (aa 1-961)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-961

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-961 truncation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 197-972

Plasmid

#111991

Purpose

Truncated Hsh155 (aa 197-972)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 197-972

Use

Tags

Expression

Bacterial and Yeast

Mutation

197-972 truncation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-945

Plasmid

#112002

Purpose

Truncated Hsh155 (aa 1-945)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-945

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-945 truncation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Hsh155 1-910

Plasmid

#112003

Purpose

Truncated Hsh155 (aa 1-910)

Depositor

Aaron Hoskins

Article

Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

Insert

Hsh155 1-910

Use

Tags

Expression

Bacterial and Yeast

Mutation

1-910 truncation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p414GPD_Hsp82viiB

Plasmid

#61709

Purpose

Yeast plasmid with Hsp82 heterodimer construct.

Depositor

Dan Bolon

Article

Mishra et al Mol Cell. 2014 Jan 23;53(2):344-50. doi:

Insert

Hsp82viiB (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Heteromeric coiled coil inserted after amino acid…

Promoter

GPD1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p414CYC_Hsp82_CycTER

Plasmid

#61713

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p414TEF_Hsp82_noTER

Plasmid

#61714

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p414ADH_Hsp82_noTER

Plasmid

#61715

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p414CYC_Hsp82_noTER

Plasmid

#61716

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Article

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

CYC1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
UPR pBARUra3

Plasmid

#45354

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

DHFR Ub Rek Ura3

Use

Tags

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
UPR pBAM Ura3

Plasmid

#45355

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

DHFR Ub Mek Ura3

Use

Tags

Expression

Yeast

Mutation

Promoter

pCUP1

Available since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pWZ414-F22

Plasmid

#23080

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 5 to Arginine, H4 K5R.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F24

Plasmid

#23081

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed Lysine 16 to Arginine, H4 K16R.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F25

Plasmid

#23082

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H4 changed lysine 16 to Glutamine, H4 K16…

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F26

Plasmid

#23083

Depositor

Sharon Dent

Article

Matangkasombut et al Mol Cell. 2003 Feb . 11(2):353-63.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone 4 changed Lysine 16 to glycine, H4 K16G.

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pRS314-prp16-1

Plasmid

#89972

Purpose

prp16-1 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp16-1 allele (Y386D)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-prp16-101

Plasmid

#89973

Purpose

prp16-101 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

prp16-101 allele (G373S)

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES942

Plasmid

#90408

Purpose

Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-SIC1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pTPGI_dCas9_VP64

Plasmid

#49013

Purpose

encodes yeast-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (codon-optimized for expression in S. cerevisiae)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

pTPGI (galactose+aTc inducible)

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS424GFP-FYVE(EEA1)

Plasmid

#36096

Depositor

Scott Emr

Article

Burd et al Mol Cell. 1998 Jul;2(1):157-62.

Insert

FYVE(EEA1) domain (EEA1 Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

PRC1

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR-MR-5Z

Plasmid

#23059

Depositor

Charles Miller

Article

Miller et al Plasmid. 2009 Dec 3. ():.

Insert

MR (NR3C2 Human)

Use

Tags

Expression

Yeast

Mutation

N528H, Q814R, H993L

Promoter

Available since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1-10

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX3X-RanBP2dFG

Plasmid

#53120

Purpose

E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

Depositor

Frauke Melchior

Article

Pichler et al Cell. 2002 Jan 11;108(1):109-20.

Insert

RanBP2 fragment (RANBP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

fragment (amino acids 2553-2838)

Promoter

tac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD-pZ-FLAG

Plasmid

#183148

Purpose

Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 BD

Use

Yeast two-hybrid

Tags

FLAG

Expression

Yeast

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3xFLAG-dCas9/pTEF1p-CYC1t

Plasmid

#62190

Purpose

Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

Insert

3xFLAG-dCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Yeast

Mutation

human codon-optimized, D10A, H840A

Promoter

TEF1 promoter

Available since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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