We narrowed to 834 results for: Anti-CRISPR

1. pcloDF-amp

   Plasmid

   #184844

   Purpose

   Backbone that confers Ampicillin resistance, contains cloDF ori

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

   Plasmid

   #115136

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

   Plasmid

   #125148

   Purpose

   Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   AcrIIA4-P2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

   Plasmid

   #115137

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

   Plasmid

   #123658

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

   Plasmid

   #115140

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

   Plasmid

   #115138

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

   Depositor

   Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA2 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CNC3G (CASANOVA-C3 with glycine linkers)

   Plasmid

   #137192

   Purpose

   Expresses CNC3G (CASANOVA-C3 with glycine linkers), a blue light dependent NmeCas9 inhibitory protein

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

   Insert

   CNC3G (CASANOVA-C3 with glycine linkers)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Keith Joung, Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPJ23116-MS2-rep

    Plasmid

    #184842

    Purpose

    Expression of the rep protein of phage MS2

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Replicase gene MS2 phage
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23116

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. P70a-deGFP-sc101-kan

    Plasmid

    #184841

    Purpose

    Expression of deGFP

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    green fluorescent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    promoter OR2-OR1

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1148-pCSDest-AcrIIA4-mCherry

    Plasmid

    #126204

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1146-pCSDest-AcrIIC3Nme-mCherry

    Plasmid

    #126202

    Purpose

    Mammalian expression of AcrIIC3Nme fused to mCherry

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIC3Nme
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

    Plasmid

    #123657

    Purpose

    DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

    Depositor

    Stanley Qi

    Article

    Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

    Insert

    mCherry-P2A-DD-AcrIIA4
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    mCherry-P2A

    Expression

    Mammalian

    Promoter

    CMV and mU6

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AcrIIC1X* (AcrIIC1X-mCherry chimera)

    Plasmid

    #128114

    Purpose

    Expresses AcrIIC1(N3F/D15Q/A48I) fused to mCherry; mediates potent inhibition of both, N. meningitidis as well as S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX* (AcrIIC1 N3F/D15Q/A48I fused to mCherry)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD2 SV40-AcrIIA4 (GB3333)

    Plasmid

    #160605

    Purpose

    SV40-AcrIIA4 codon optimized for N.benthamiana

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

    Insert

    AcrIIA4
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS

    Plasmid

    #126203

    Purpose

    Mammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIC3Nme
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1149-pCSDest-AcrIIA4-mCherry-miR122BS

    Plasmid

    #126205

    Purpose

    Mammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    codon-optimized AcrIIA4
    

    Tags

    FLAG/NLS and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid

    #133794

    Purpose

    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    NmeCas9 and Nme2Cas9 sgRNA entry vector
    

    Expression

    Mammalian

    Mutation

    sgRNA architecture from Amrani et al. Genome Biol…

    Promoter

    U6

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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