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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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HUW-TetO-hNanog

Plasmid

#25700

Depositor

Rudolf Jaenisch

Article

Hanna et al Nature. 2009 Dec 3. 462(7273):595-601.

Insert

Nanog (NANOG Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pcF-1

Plasmid

#131023

Purpose

Single spacer array with spacer #1 for FnCas12a.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Insert

single spacer array for FnCas12a

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PJ23119

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pRL1124

Plasmid

#70690

Purpose

provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.

Depositor

Peter Wolk

Article

Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Inserts

avaiM
eco47iiM
ecot22iM

Use

Bacterial shuttle vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pJZ001

Plasmid

#120230

Purpose

Contains intB13 integrase casette for knock-in to pCAP-BAC backbone

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Insert

intB13 integrase casette

Use

Tags

Expression

Bacterial

Mutation

bla-g717a (amp resistance gene)

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-T7-VHH-GFPminimizer

Plasmid

#202488

Purpose

Expresses the nanobody GFP minimizer with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody GFP minimizer

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH72

Plasmid

#202467

Purpose

Expresses the nanobody VHH72 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody VHH72

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-H6antivenin

Plasmid

#202470

Purpose

Expresses the nanobody H6 antivenin after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody H6 antivenin

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-1B5

Plasmid

#202472

Purpose

Expresses the nanobody 1B5 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 1B5

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-2E7

Plasmid

#202473

Purpose

Expresses the nanobody 2E7 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 2E7

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-3E3

Plasmid

#202474

Purpose

Expresses the nanobody 3E3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody 3E3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-C9BoNT-A

Plasmid

#202475

Purpose

Expresses the nanobody C9 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody C9 BoNT/A

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-E7BoNT-A

Plasmid

#202476

Purpose

Expresses the nanobody E7 BoNT-A after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody E7 BoNT/A

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-JM3

Plasmid

#202477

Purpose

Expresses the nanobody JM3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody JM3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-J3

Plasmid

#202478

Purpose

Expresses the nanobody J3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody J3

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-m36.4

Plasmid

#202479

Purpose

Expresses the nanobody m36.4 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody m36.4

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re5D06

Plasmid

#202480

Purpose

Expresses the nanobody Re5D06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re5D06

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re9F06

Plasmid

#202481

Purpose

Expresses the nanobody Re9F06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re9F06

Use

Tags

6X-His

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-ppiB

Plasmid

#202482

Purpose

Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

peptidyl-prolyl cis-trans isomerase (ppiB)

Use

Tags

Expression

Bacterial

Mutation

Promoter

yibDp

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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