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We narrowed to 11,242 results for: VARS

Showing: 161 - 180 of 11242 results

  1. HUHe variant click editor (CE1) - pCMV-T7-GuCV-nCas9-EcKlenow (JO1279)

    Plasmid
    #217788
    Purpose
    Variant CE1 construct with GuCV HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    GuCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217788-plasmid-map-sequence-id-432851

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  2. HUHe variant click editor (CE1) - pCMV-T7-PCV1-nCas9-EcKlenow (JO1315)

    Plasmid
    #217789
    Purpose
    Variant CE1 construct with PCV1 HUH endonuclease, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV1-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217789-plasmid-map-sequence-id-432852

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  3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

    Plasmid
    #208957
    Purpose
    A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208957-plasmid-map-sequence-id-415746

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  4. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

    Plasmid
    #208958
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208958-plasmid-map-sequence-id-415784

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  5. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  6. pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant

    Plasmid
    #101208
    Purpose
    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A
    Use
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A and Q695A
    Promoter
    T7
    Available Since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101208-plasmid-map-sequence-id-198110

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  14. pRD60 (dE2F)

    Plasmid
    #14820
    Depositor
    Robert Duronio
    Insert
    E2F (E2f1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 30, 2007
    Availability
    Academic Institutions and Nonprofits only

  15. E2F1 in pGEX4t

    Plasmid
    #112976
    Purpose
    For protein expression and purification of Drosophila E2F1
    Depositor
    Terry Orr-Weaver
    Insert
    E2F1 (E2f1 Fly)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    112976-plasmid-map-sequence-id-217467

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  16. pSBBi-RP PcCS

    Plasmid
    #139159
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    Caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    139159-plasmid-map-sequence-id-309125

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  17. pSBBi-RP GST-PcCS

    Plasmid
    #139160
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes.
    Depositor
    Neal Devaraj
    Insert
    N-GST caffeine Synthase [Paullinia cupana var. sorbilis]
    Use
    Transposon
    Tags
    glutathione transferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available Since
    Availability
    Academic Institutions and Nonprofits only
    139160-plasmid-map-sequence-id-309126

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Showing: 161 - 180 of 11242 results