We narrowed to 611 results for: umps
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Plasmid#207608PurposesgRNA 2 for homology directed repair insertion of 3xMS2-TR-100-PURO into the endogenous TR locusDepositorInsertacccccaaacctgactgact
TagsNoneExpressionMammalianPromoterCMV and U6Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR knockout HRD
Plasmid#207579PurposeHomologous recombination donor replacing TR with a puro cassette.DepositorInsertpuro resistance cassette flanked by TR locus sequences excluding TR
TagsNoneExpressionMammalianPromoterSV40Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA
Plasmid#220221PurposeAAV-STARR-seq validation vector with GFPDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-UDP1.0-IRES-mCherry-CAAX
Plasmid#220850PurposeCMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherryDepositorInsertUDP1.0
ExpressionMammalianPromoterCMVAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-GluR1Ctail
Plasmid#32441DepositorInsertGluR1 (GRIA1 Human)
TagsEGFPExpressionMammalianMutationContains the last C-terminal 81 amino acids of th…PromoterCMVAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
TagsmCherryExpressionMammalianPromoterLTRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-N1-eKR2
Plasmid#115337PurposeExpresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouseDepositorInserteKR2
TagsEYFPExpressionMammalianMutationnonePromoterCMVAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA
Plasmid#220224PurposeAAV-STARR-seq validation control vector with VenusDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA
Plasmid#220222PurposeAAV-STARR-seq validation vector with RFPDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
Tags3xMS2ExpressionMammalianPromoterSV40Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA
Plasmid#220223PurposeAAV-STARR-seq validation control vector with TFPDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_SC_4.26_STARR-seq
Plasmid#220220PurposeAAV-STARR-seq screening vector with 4.26 promoteDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoter4.26Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-UDP1.0-WPRE
Plasmid#220849PurposeAAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)DepositorInsertUDP1.0
UseAAVPromoterhuman Synapsin (hSyn)Available SinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA
Plasmid#220225PurposeAAV-STARR-seq validation vector containing VS33 enhancerDepositorInsertEnhancer (VS33)
UseAAVExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
ExpressionBacterialPromoterGlnS (constitutive)Available SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialPromoteraraC and lppAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP WT
Plasmid#197567PurposeExpression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)
TagsV5-His6ExpressionMammalianPromoterCMV and U6/H1Available SinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP 150TAG
Plasmid#197574PurposeFluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.DepositorInsertssfGFP 150TAG
M. alvus Pyl-tRNA (6)
TagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only