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M71::GFP LTNL TV

Plasmid

#15618

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

M71::GFP LTNL TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
1162BY-CMV-mCherry-bGHpolyA

Plasmid

#235172

Purpose

Expresses mCherry

Depositor

Omar Akbari

Insert

mCherry

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_LD 0_CS G_tTA-BFP

Plasmid

#58876

Purpose

MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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3538 pMIG Bad

Plasmid

#8787

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bad (Bad Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2005

Availability

Academic Institutions and Nonprofits only
EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Myc-Xdsh-pCS2 (RXF)

Plasmid

#15490

Depositor

Sergei Sokol

Article

Sokol et al Curr Biol. 1996 Nov 1. 6(11):1456-67.

Insert

Dishevelled (dvl2.L Frog)

Tags

myc

Expression

Mammalian

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2TK E7 del EDE

Plasmid

#13675

Depositor

Karl Munger

Article

Jones et al Genes Dev. 1997 Aug 15. 11(16):2101-11.

Insert

HPV 16 E7 del EDE (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

del aa 35-37

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

Plasmid

#186980

Purpose

mammalian expression of Cluc-Gluc reporter

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

Cluc, Gluc

Use

Luciferase

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)

Plasmid

#63139

Purpose

Expression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)

Depositor

Titia Sixma

Article

Buchwald et al EMBO J. 2006 Jun 7;25(11):2465-74. Epub

Inserts

Ring1b (Ring1 Mouse)
Bmi1 (Bmi1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-109 and Residues 1-159

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-REDD1-HA

Plasmid

#133550

Purpose

Expresses HA tagged REDD1 in Mammalian cells

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

Insert

Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA5-mRuby3

Plasmid

#184891

Purpose

Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA5 (ANXA5 Human)

Tags

mRuby3

Expression

Mammalian

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr Y205A

Plasmid

#102438

Purpose

cAMPr with a point mutation Y205A which should render PKA-C catalytically dead

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

PKA-C Y205A

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDBTrp-LexABD-CRY2FL

Plasmid

#78210

Purpose

Use with Addgene 40615 for blue light transcription control in yeast

Depositor

Chandra Tucker

Article

Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

Insert

LexABD-CRY2 (CRY2 Mustard Weed)

Tags

lexA binding domain

Expression

Yeast

Mutation

contains endogenous CRY2 NLS

Promoter

ADH1

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCri-18a

Plasmid

#61326

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-invitro(KaganD86)

Plasmid

#52012

Purpose

Used for in vitro expression of the human MAVS CDS

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50004

Plasmid

#50316

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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