Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 1,853 results for: FAST

Showing: 1781 - 1800 of 1853 results

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pFB HTB_hMSH6_T1219D

Plasmid

#135466

Purpose

Expression human MSH6_T1219D mutant protein using baculovirus

Depositor

Peggy Hsieh

Publication

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6_T1219D (MSH6 Human)

Tags

His-tag

Expression

Insect

Mutation

changed threonine to aspartic acid

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF2A:1-427

Plasmid

#210939

Purpose

Insect expression of human EIF2A fragment M1 to T427. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF2A:M1-T427 (EIF2A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_IFT172:1-320

Plasmid

#210934

Purpose

Insect expression of human IFT172 fragment M1 to S320. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

IFT172:M1-S320 (IFT172 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_TRAF7:382-670

Plasmid

#210930

Purpose

Insect expression of human TRAF7 fragment P382 to C670. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

TRAF7:P382-C670 (TRAF7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO7:1-925

Plasmid

#210921

Purpose

Insect expression of full-length human CORO7. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO7:M1-D925 (CORO7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_ATG16L2:321-619

Plasmid

#210918

Purpose

Insect expression of human ATG16L2 fragment A321 to Q619. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L2:A321-Q619 (ATG16L2 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_UTP4:1-686

Plasmid

#210915

Purpose

Insect expression of full-length human UTP4. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP4:M1-R686 (CIRH1A Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_DCAF13:54-356

Plasmid

#210908

Purpose

Insect expression of human DCAF13 fragment F54 to A356. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF13:F54-A356 (DCAF13 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

A86S sequence conflict

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR12:90-417

Plasmid

#210903

Purpose

Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-T417 (WDR12 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

introduced mutations F99A K131A R319S

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RACK1:1-317

Plasmid

#210890

Purpose

Insect expression of full-length human RACK1. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RACK1:M1-R317 (RACK1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_SMU1:170-513

Plasmid

#210882

Purpose

Insect expression of human SMU1 fragment K170 to P513. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SMU1:K170-P513 (SMU1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR91:392-747

Plasmid

#210878

Purpose

Insect expression of human WDR91 fragment P392 to A747. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:P392-A747 (WDR91 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR12:90-422

Plasmid

#210893

Purpose

Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR12:T90-G422 (WDR12 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR41:30-459

Plasmid

#210892

Purpose

Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-V459 (WDR41 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_WDR91:1-747

Plasmid

#210879

Purpose

Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR91:M1-A747 (WDR91 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Chinchilla+N122D–insM

Plasmid

#191227

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 and replaced asparagine…

Promoter

PH and p10

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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S+Q111R+N122D

Plasmid

#167175

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R and N122D mutations in Sf9 cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+Q111R+N122D
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed Q at 111 to R and changed N at 122 to D

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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mWasabi-ADAP1

Plasmid

#163906

Purpose

Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

Depositor

Martin Loose

Publication

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

ADAP1 (Adap1 Mouse)

Tags

mWasabi fluorescent tag

Expression

Insect

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt13C(WT)

Plasmid

#86576

Purpose

Expresses cleavable human full-length Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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3xMS-TR-100-PURO HRD

Plasmid

#207582

Purpose

homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences

Tags

3xMS2

Expression

Mammalian

Promoter

SV40

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Showing: 1781 - 1800 of 1853 results

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