We narrowed to 2,463 results for: T2A

1. eGFP L202 Reporter

   Plasmid

   #119129

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Mutation

   eGFP L202S

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p160Tol2-her4.3:switchCas9

   Plasmid

   #227680

   Purpose

   Expression of cre inducible and her4.3 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs for SABER-seq in zebrafish

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   loxP-dsRed-loxP-Cas9-t2A-GFP
   4xU6:sgRNA
   

   Use

   CRISPR

   Promoter

   U6 and her4.3

   Available Since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP L138 Reporter

   Plasmid

   #119130

   Purpose

   Acts as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   mCherry and eGFP
   

   Use

   Lentiviral

   Mutation

   eGFP L138S

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-PTE2A

   Plasmid

   #111773

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

   Plasmid

   #227775

   Purpose

   Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

   Depositor

   Bushra Raj

   Article

   Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

   Inserts

   BFP-MCS
   mCherry-t2A-CreERT2
   

   Use

   Zebrafish expression

   Promoter

   Olactb and hsp70l

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-sfGFP-3xP3-RFP

   Plasmid

   #127566

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Expression

   Mammalian

   Mutation

   A206K both

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

   Plasmid

   #194880

   Purpose

   Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

   Depositor

   Peter Scheiffele

   Article

   Czernecki et al bioRxiv 2022.11.16.516849

   Insert

   GFP-T2A-mCherry-D4H
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   human Synapsin

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. eGFP Y93H TC editing reporter

   Plasmid

   #198882

   Purpose

   Express eGFP fluorescence after Y93H correction and mCherry as transfection control

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   enhanced green fluorescent protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Y93H

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNW3

    Plasmid

    #53372

    Purpose

    Csy4 and Cas9 D10A nickase expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9n (D10A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV hSynapsin SV-tag

    Plasmid

    #163685

    Purpose

    express SV-Tag construct in a Cre-independent manner

    Depositor

    Bernardo Sabatini

    Article

    Chantranupong et al Elife. 2020 Oct 12;9. pii: 59699. doi: 1

    Insert

    synatophysin 9X HA tag T2A tdtomato
    

    Use

    AAV

    Tags

    9X HA

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEntry Tshld(FKBP F36V)-GFP

    Plasmid

    #53260

    Purpose

    pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

    Depositor

    Matthew Pratt

    Article

    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

    Insert

    Tshld(FKBP F36V)-GFP
    

    Tags

    EGFP, Flag, and HA

    Mutation

    FKBP F36V

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQP-AR10

    Plasmid

    #138980

    Purpose

    Endogenous RAS regulation using light-controlled intrabody

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    BglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
    

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SEPLuc

    Plasmid

    #141102

    Purpose

    bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc
    

    Use

    Lentiviral

    Promoter

    EF1α

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-DiO-eGFP-2A-mCherry_YDA

    Plasmid

    #194881

    Purpose

    Ratiometric YDA sensor for in vivo cre-dependent neuron-specific cholesterol visualization

    Depositor

    Peter Scheiffele

    Article

    Czernecki et al bioRxiv 2022.11.16.516849

    Insert

    GFP-T2A-mCherry-YDA
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PYPQ121-DdCBE

    Plasmid

    #218174

    Purpose

    Express plastid DdCBE, composed of TALE, split DddA originated from Burkholderia cenocepacia and UGIs

    Depositor

    Yiping Qi

    Article

    Wang et al Plant Biotechnol J. 2024 May 6. doi: 10.

    Insert

    TALE-Intron-N-DddAtox half (G1397N)-UGI-T2A-TALE-C-DddAtox half (G1397C)-UGI
    

    Use

    Plastid base editing

    Expression

    Plant

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. TC562

    Plasmid

    #164887

    Purpose

    Punc-4::rtetR-QFAD(650-777+790-816)::P2A::mKate::T2A::tetR-pie1

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    QFAD mutated
    

    Expression

    Worm

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK591

    Plasmid

    #71589

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine and aspartate-177 …

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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