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pXD80ENSF8-HcdhA(cHis8)BC-HcdR-Accs(ElA2)

Plasmid

#225303

Purpose

Expresses Eggerthella lenta A2 Hcdh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh

Tags

His8

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF18-C1878_02750_GsVadh(Gs28C)

Plasmid

#225304

Purpose

Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Gs Vadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

Plasmid

#225311

Purpose

Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN4-Elen0511-(–)-Eldh1-noAccs(ElA2)

Plasmid

#225312

Purpose

Expresses Eggerthella lenta A2 (–)-Eldh1 without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 )

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

Plasmid

#225298

Purpose

Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70EN25-Pct5-(–)-Eddh(Gp3C)

Plasmid

#233877

Purpose

Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Article

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-HIS3MX6

Plasmid

#227975

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FRB-HA3

Plasmid

#228008

Purpose

Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CUP1p-TID-FRB-NLS-HA3

Plasmid

#228009

Purpose

Expresses TurboID-FRB-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CUP1p-TID-FKBP-HA3

Plasmid

#228010

Purpose

Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CUP1p- TID-FKBP-NLS-HA3

Plasmid

#228011

Purpose

Expresses TurboID-FKBP-NLS fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CHA1p-TID-FRB-HA3

Plasmid

#228012

Purpose

Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p-TID-FRB-NLS-HA3

Plasmid

#228013

Purpose

Expresses TurboID-FRB-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CHA1p- TID-FKBP-NLS-HA3

Plasmid

#228015

Purpose

Expresses TurboID-FKBP-NLS fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BioID2-HA3-HIS3MX6

Plasmid

#227977

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID3

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-BASU-HA3-HIS3MX

Plasmid

#227979

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-TID-FRB-HA3

Plasmid

#227996

Purpose

Expresses TurboID-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-KanMX6

Plasmid

#228020

Purpose

For TurboID-FRB integration; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFa6-TID-FRB-3HA-HIS3MX6

Plasmid

#228021

Purpose

For TurboID-FRB integration; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge