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FU-MAP2-Gateway

Plasmid

#43915

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector

Tags

V5 and non-standard His tag (HHRHHH)

Expression

Mammalian

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-DDK

Plasmid

#137764

Purpose

Control plasmid for immunoprecipitation of DDK/FLAG-tagged proteins. Also a good control for transfection efficiency.

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GFP

Tags

DDK

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-OCT4

Plasmid

#69537

Purpose

Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

5 concatenated gRNA transcriptional cassettes targeting OCT4

Use

CRISPR

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Expression

Mammalian

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
pBEG R2-L3 #BGV193

Plasmid

#48952

Purpose

For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
Cenp-B DBD INCENP GFP

Plasmid

#45237

Purpose

Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

Cenp-B-DBD, INCENP-Δcen, GFP

Tags

DNA binding domain of CENP-B and GFP

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRN3P_T3_BE4_IVT

Plasmid

#171762

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the BE4 base editor (C to T) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

BE4

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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MLSE-shREN

Plasmid

#105583

Purpose

retrovirally express control shRNA with GFP marker

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

control shRNA targeting luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-HA.Tetherin

Plasmid

#41069

Depositor

Paul Spearman

Article

Hammonds et al PLoS Pathog. 2010 Feb 5;6(2):e1000749.

Insert

Tetherin (BST2 Human)

Use

Retroviral

Tags

HA and IRES Puro

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
KG#121

Plasmid

#110879

Purpose

Expresses the C. elegans unc-31 cDNA pan-neuronally

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

rab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Mutation

Missing first 408bp

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR (GB2566)

Plasmid

#160595

Purpose

Minimal promoter of slDFR gene containing 62bp upstream the transcription start site and the 5'UTR region

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-U6c

Plasmid

#107599

Purpose

An entry vector with U6a and U6c promoter driving guide RNAs expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Inserts

U6a promoter
U6c promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4

Plasmid

#220451

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4DBD

Plasmid

#220452

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-Gal4DBD

Plasmid

#220454

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(1)-p65AD

Plasmid

#220456

Purpose

Split intein version of Trojan-p65AD. Create p65AD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-p65AD-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(0)-Gal4

Plasmid

#220449

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 0 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Expression

Insect

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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psiTE(2)-LexAGAD

Plasmid

#220460

Purpose

Split intein version of Trojan-LexAGAD. Create LexAGAD driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 2 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-LexAGAD-CfaC

Expression

Insect

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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