We narrowed to 11,141 results for: hal

1. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

   Plasmid

   #198599

   Purpose

   Green fluorescent Ca2+ ion indicator for bacterial expression.

   Depositor

   Robert Campbell

   Article

   Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

   Insert

   HaloGFP-143-144
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Halotag-loxP-P2A-dTKneo-pA-loxP

   Plasmid

   #139485

   Purpose

   PCR template for Halo-Tag gene knock-in donor vector

   Depositor

   Kazuhiro Aoki

   Article

   Komatsubara et al J Biol Chem. 2019 Apr 12;294(15):6062-60

   Insert

   HaloTag-loxP-P2A-dTKneo-pA-loxP
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223 MitoFlux (pSu9-HaloTag7-mGFP-STOP)

   Plasmid

   #215001

   Purpose

   Shuttling vector for a mitophagy reporter

   Depositor

   Wade Harper

   Insert

   pSu9 (Presequence of Fo-ATPase subunit 9)
   

   Use

   Gateway

   Tags

   HaloTag(7)-mGFP

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP-Puro-TetOn-L1RP-ORF1_K3AK4A_Halo-GFPai

   Plasmid

   #202568

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the K3A and K4A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Lysine 3 to Alanine, ORF1p Lysine 4…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP)

   Plasmid

   #215003

   Purpose

   Shuttling vector for a Lysophagy reporter

   Depositor

   Wade Harper

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Gateway

   Tags

   HaloTag(7)-mGFP

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXS-IRES-BLAST Halo-flag-CAD

   Plasmid

   #188117

   Purpose

   Expresses N-terminal Halo-tagged/C-terminal flag-tagged human CAD in mammalian cells

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag and Halo tag

   Expression

   Mammalian

   Mutation

   TCC -> AGT silent mutations at nt439-441 elimi…

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

   Plasmid

   #134612

   Purpose

   Mammalian expression of mouse KIF5B 376-963

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5B (Kif5b Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 375 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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