We narrowed to 14,103 results for: NTS

1. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214280

   Purpose

   Express split-Rac1 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   Rac1-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

   Plasmid

   #214281

   Purpose

   Express split-Rac1 fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   Rac1-T17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

   Plasmid

   #214283

   Purpose

   Express split-RhoA fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   RhoA-T19N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLT3REVIR_MARK3 2573

   Plasmid

   #107233

   Purpose

   shRNA 2573. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3 shRNA (22 nt) (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLT3REVIR_MARK3 1122

   Plasmid

   #107232

   Purpose

   shRNA 1122. Do not use Evos or Acurri on these cells. Assess by FACS specifically with Venus and DsRed lazers. Tet-ON-all-in-one

   Depositor

   Alex Kentsis

   Article

   Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

   Insert

   MARK3 shRNA (22 nt) (MARK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(Q61L)-FKBP-mVenus-CAAX

   Plasmid

   #214284

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIRES-mCerulean-FRB-KRas/N12-KRas/13C(S17N)-FKBP-mVenus-CAAX

   Plasmid

   #214285

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(Q61L)-GAI1-92-mVenus-CAAX

   Plasmid

   #214286

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (N terminal on insert 1) and mVenus (on…

   Expression

   Mammalian

   Mutation

   KRas-Q61L

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIRES-mCerulean-GID1-KRas/N12-KRas/13C(S17N)-GAI1-92-mVenus-CAAX

   Plasmid

   #214287

   Purpose

   Express split-KRas fragments that are fused with CIDs and FPs

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Inserts

   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   Split-KRas fragments fused with CIDs and FPs (KRAS Human)
   

   Use

   Tags

   mCerulean (on insert 1) and mVenus (on insert 2)

   Expression

   Mammalian

   Mutation

   KRas-S17N

   Promoter

   CMV

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7NT*-Bri2 113-231 R221E

    Plasmid

    #138134

    Purpose

    Expresseds human Bri2 BRICHOS R221E mutant in E. coli

    Depositor

    Jan Johansson

    Article

    Chen et al Commun Biol. 2020 Jan 20;3(1):32. doi: 1

    Insert

    human Bri2 BRICHOS R221E (ITM2B Human)
    

    Use

    Tags

    NT* tag derived from spider silk proteins

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. L40C-CRISPR.EFS.mNeon

    Plasmid

    #69146

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLKO.3 Thy1.1

    Plasmid

    #14749

    Depositor

    Christophe Benoist, Diane Mathis

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.3G

    Plasmid

    #14748

    Depositor

    Christophe Benoist, Diane Mathis

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ352

    Plasmid

    #30539

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCFJ210

    Plasmid

    #30538

    Depositor

    Erik Jorgensen

    Insert

    ttTi4348 region
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSARGENT1

    Plasmid

    #216315

    Purpose

    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence

    Depositor

    Barak Cohen

    Insert

    tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SnFR_S074

    Plasmid

    #182811

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S074-CholineSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SnFR_S062

    Plasmid

    #182810

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Insert

    S062-CholineSnFR
    

    Use

    Tags

    6xHis, HA and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CAMP no 3'UTR

    Plasmid

    #129561

    Purpose

    cytomplasmic marker without terminator (less integration)

    Depositor

    Inaki Ruiz-Trillo

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge