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Plasmid#49333PurposeRepaired Addgene #26576 to agree with the rat ref sequence. Repairs are @: nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
UseTagsExpressionMammalianMutationContains exons 8a, 11, 31b and 42a.Deletion of ex…PromoterCMVAvailable sinceFeb. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA - Exon9 E545K
Plasmid#16642DepositorInsertPIK3CA E545K (PIK3CA Human)
UseTagsFlagExpressionMammalianMutationE545K; see note belowPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
ACTN3-pEGFP
Plasmid#52670PurposeOverexpression of GFP-tagged α-actinin-3DepositorInsertACTN3 (ACTN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pM2AH + mSlo1
Plasmid#16152DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIIα-Dronpa
Plasmid#182331PurposePKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.DepositorInsertRIIalpha-Dronpa (PRKAR2A Human)
UseTagsFull-length human PKA type 2alpha regulatory subu…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CD8-GFP-FKBP
Plasmid#100751PurposeExpression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.DepositorInsertCD8A (CD8A Human)
UseTagsFKBP and GFPExpressionMammalianMutationTruncatedPromoterCMVAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_A
Plasmid#74374PurposegRNA_A to knockout human AMPK alpha 1 using Cas9nDepositorInserthuman AMPK alpha 1 exon1 gRNA (PRKAA1 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dox-Akt
Plasmid#207328PurposePiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)DepositorInsertrac protein kinase-alpha (AKT1 Human)
UseTagsmyristoylation tag - BFPExpressionBacterialMutationrange: 129 - 480PromoterTet Response ElementAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GFP-IRES-mSNCA
Plasmid#185714PurposeAAV expression of GFP and mouse α-Synuclein from hSyn promoterDepositorInsertsynuclein, alpha (Snca Mouse)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorUseTagsExpressionInsectMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pECE HA AMPKa1 AS
Plasmid#69831PurposeExpresses Expresses AMPK alpha 1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseTagsHAExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP-shACTN1
Plasmid#52676PurposeshRNA against α-actinin-1 with eGFP transfection markerDepositorInsertACTN1 (Actn1 Rat)
UseRNAiTagsCMV-EGFPExpressionMutationPromoterU6Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP-shACTN4
Plasmid#52679PurposeshRNA against α-actinin-4 with eGFP transfection markerDepositorInsertACTN4 (Actn4 Rat)
UseRNAiTagsCMV-EGFPExpressionMutationPromoterU6Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSIL-eGFP-shACTN2
Plasmid#52677PurposeshRNA against α-actinin-2 with eGFP transfection markerDepositorInsertACTN2 (Actn2 Rat)
UseRNAiTagsCMV-EGFPExpressionMutationPromoterU6Available sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S87A
Plasmid#36056DepositorInsertalpha-synuclein S87A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS87APromoterCMVAvailable sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-TUBA1B_sgRNA
Plasmid#183888PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1E-1
Plasmid#21193DepositorUseRetroviralTagsExpressionMutationMek1 R4F fused to ER*, constitutive activePromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
TMED9-bio-His
Plasmid#51855PurposeExpresses full-length Transmembrane emp24 domain-containing protein 9 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTMED9 (TMED9 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX p85a(C)-SH2
Plasmid#46463DepositorInsertp85a(C) (PIK3R1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 612-724PromoterTacAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only