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pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221pmCDA1-T7RNAP
Plasmid#167978PurposeExpresses fusion protein pmCDA1-T7RNAP in E. coliDepositorInsertpmCDA1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS YFP
Plasmid#91759PurposeBacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)DepositorInsertVenus YFP (A206K)
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 NM23-H1
Plasmid#10956DepositorInsertNM23 (NME1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-null-GFPLVA
Plasmid#23338DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-smURFP-IRES-eGFP
Plasmid#80343PurposeExpresses smURFP and eGFP in mammalian cells.DepositorInsertssmURFP
eGFP
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ConVERGD-eGFP-WPRE
Plasmid#218749PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL
Plasmid#218750PurposeProvides NOT intersectional (Flp NOT Cre) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9
Plasmid#64237Purposeexpresses codon optimized Cas9 in zebrafishDepositorInsertcodon optimized Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGal4AD-CIBN
Plasmid#28246PurposeExpresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activationDepositorInsertCIB1 (1-171aa)
UseTagsGal4ADExpressionYeastMutationPromoterAvailable sinceMarch 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pSEVA221rAPOBEC1-T7RNAP
Plasmid#167979PurposeExpresses fusion protein rAPOBEC1-T7RNAP in E. coliDepositorInsertrAPOBEC1-T7RNAP (T7p07 Synthetic)
UseTagsT7 tag and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-ChETA-EYFP
Plasmid#26967Purpose3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activationDepositorInserthChR2(E123T/H134R)-EYFP
UseLentiviralTagsEYFPExpressionMammalianMutationE123TPromoterCaMKIIaAvailable sinceJan. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pU6a:sgRNA(tyr)
Plasmid#64250Purposeexpresses sgRNA(tyr) under U6a promoterDepositorInsertU6a:sgRNA (tyr)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
C413-E45: attB4-hr5-ie1p>-attB1r
Plasmid#162930PurposeGateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPVDepositorInsertAcMNPV ie1 promoter with hr5 enhancer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-G12-mTGFB1
Plasmid#219553PurposemTGFB1 expression in mammlian cells. pAAV vector. Reporter plasmid.DepositorInsertmTGFB1 (Tgfb1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoter12xUAS with TATA-box minimal promoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BH*deltavpu/RT-
Plasmid#136985PurposeSecond-generation lentiviral packaging plasmid lacking reverse transcriptase activityDepositorInsertHIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
UseTagsExpressionMammalianMutationcarries D110E mutation in the reverse transcripta…PromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJT119b
Plasmid#50551PurposeExpresses CcaS and CcaR constitutively, and sfGFP under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-ConVERGD-eGFP-WPRE
Plasmid#218747PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoternEFAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-ConVERGD-eGFP-W3SL
Plasmid#218746PurposeProvides AND intersectional (Cre+Flp) expression of eGFPDepositorInserteGFP
UseAAVTagsExpressionMutationPromoternEFAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYC56
Plasmid#63910PurposeRecipient integrative vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterAvailable sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PssaG-GFPLVA
Plasmid#23343DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC3-C2cd3
Plasmid#128871PurposeFor mammalian expression of mouse C2cd3DepositorInsertC2cd3 (C2cd3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYC34
Plasmid#63915PurposeRecipient overexpressing vector to construct mCherry tagged fusionsDepositorTypeEmpty backboneUseTagsmCherryExpressionYeastMutationPromoterTEF1 (Saccharomyces cerevisiae)Available sinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN-mEGFP-C1
Plasmid#110468Purposeretrovirus construct for stable expressing mEGFP tagged proteinDepositorInsertmEGFP
UseRetroviral; RetrovirusTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only
