We narrowed to 5,521 results for: cycs

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pBluescript goosecoid

Plasmid

#16935

Depositor

John Postlethwait

Article

Thisse et al Dev Biol. 1994 Aug . 164(2):420-9.

Insert

goosecoid (gsc Zebrafish)

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pCB6HindIII-rat FcRn

Plasmid

#121671

Purpose

Expresses rat neonatal Fc receptor in mammalian cells

Depositor

Pamela Bjorkman

Article

Tesar et al Traffic. 2006 Sep;7(9):1127-42.

Insert

rat neonatal Fc receptor (Fcgrt Rat)

Expression

Mammalian

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMA2867

Plasmid

#25439

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

mCherry-miR155-lacZalpha (Mir155hg Mouse, Discosoma-mouse-E. coli)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pPEE17

Plasmid

#128361

Purpose

Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

Depositor

James Fraser

Article

Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

Insert

Acetamidase (amdS)

Use

Unspecified

Promoter

TEF1

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#53300

Purpose

Contains Erwinia herbicola Eho10 (Pantoea agglomerans) crtE and crtB genes, and thereby produces phytoene in E. coli

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.

Insert

crtE, crtB

Use

Low copy number bacterial cloning vector

Promoter

endogenous promoters

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pADH139

Plasmid

#90987

Purpose

NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR; use with pADH110 to generate target-specific part 2 of 2 for C.mal LEUpOUT CRISPR system.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

gRNA conserved, C, mal LEU2 2 of 2

Use

CRISPR

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRSV-p65S276A

Plasmid

#106455

Purpose

NF-kB p65, kinase site 276 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 276 mutated (RELA Human)

Use

Lentiviral

Expression

Mammalian

Mutation

kinase site inactivated

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSR33

Plasmid

#69254

Purpose

phsp16.2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

hsp16.2

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MKRN1_H307E

Plasmid

#78756

Purpose

To overexpress MKRN1_H307E in Mammalian Cell

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H307E

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAPM-D4-miR30-TRIM5

Plasmid

#132932

Purpose

Lentivector for TRIM5 knockdown and puromycin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

TRIM5 shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta12

Plasmid

#106116

Purpose

Lentiviral expression of PRKRA delta 12

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Mutation

Truncation

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_PRKRA_delta3

Plasmid

#106115

Purpose

Lentiviral expression of PRKRA delta3

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK and myc

Mutation

Truncation

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-THT III

Plasmid

#55791

Purpose

GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance

Depositor

Stephan Geley

Article

Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

Type

Empty backbone

Use

RNAi; Gateway

Expression

Mammalian

Promoter

modified H1 RNA gene

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pUASTattb-cAMPr Y205A, R626A

Plasmid

#84932

Purpose

cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr Y205A R626A

Expression

Insect

Mutation

PKA-C Y205A, PKA-R R626A

Promoter

5X UAS

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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SEPT5 Mut(S161A/S327A)

Plasmid

#27275

Depositor

Harish Pant

Article

Amin et al J Neurosci. 2008 Apr 2. 28(14):3631-43.

Insert

Septin 5 (SEPTIN5 Human)

Tags

Myc (MASMQKLISEEDL)

Expression

Mammalian

Mutation

S161A and S327A

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJK357

Plasmid

#71568

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

succinyl-CoA:acetate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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