Skip to main content
Addgene
Showing: 1801 - 1820 of 29406 results

  2. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Article
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105627-plasmid-map-sequence-id-207217

    Enlarge

  12. pUC57-BBclone1

    Plasmid
    #137069
    Purpose
    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Inserts
    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    Use
    Tags
    Expression
    Bacterial
    Mutation
    wild-type, full-length (signal-peptide-containing…
    Promoter
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137069-plasmid-map-sequence-id-275695

    Enlarge

  14. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    syntaxin 1A (Stx1a Mouse)
    Use
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79571-plasmid-map-sequence-id-456519

    Enlarge

  18. pDisCoTune

    Plasmid
    #173713
    Purpose
    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
    Depositor
    Morten Norholm
    Article
    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2
    Inserts
    ERV1 (ERV1 Budding Yeast)
    PDI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    173713-plasmid-map-sequence-id-342928

    Enlarge

  19. CIBN-CAAX

    Plasmid
    #79574
    Purpose
    expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting
    Depositor
    Olof Idevall-Hagren, Pietro De Camilli
    Article
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Insert
    CIBN (CIB1 Mustard Weed)
    Use
    Tags
    C-terminal CAAX-box
    Expression
    Mammalian
    Mutation
    K93A, R94A, K106A, K107A (please see depositor co…
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79574-plasmid-map-sequence-id-314173

    Enlarge

  20. pHSG1C3

    Plasmid
    #164423
    Purpose
    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    164423-plasmid-map-sequence-id-338872

    Enlarge

Showing: 1801 - 1820 of 29406 results