We narrowed to 30,996 results for: uros

1. pLKO.1-puro U6 sgRNA Oct4A -12

   Plasmid

   #50921

   Purpose

   U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   Oct4A -12 sgRNA (POU5F1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

   Plasmid

   #196197

   Purpose

   Cas9 + gRNA expression to edit G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-Neuropeptide Y [L115/13R-2b]

   Plasmid

   #206639

   Purpose

   Mammalian Expression Plasmid of anti-Neuropeptide Y (Human). Derived from hybridoma L115/13-2b.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Neuropeptide Y (Homo sapiens) recombinant Mouse monoclonal antibody (NPY Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W

   Plasmid

   #105040

   Purpose

   Lentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Tsc2 (Tsc2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-BE3RA-P2A-Puro

   Plasmid

   #110839

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-C-moxGFP-Puro-H3C2

   Plasmid

   #207783

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pBabe puro RalA (long) wt

   Plasmid

   #19717

   Depositor

   Channing Der

   Article

   Lim et al Curr Biol. 2006 Dec 19. 16(24):2385-94.

   Insert

   V-ral simian leukemia viral oncogene homolog A (RALA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   NOTE: long splice variant with 3bp deletion and 1…

   Promoter

   Available since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

8. tet-pLKO shMKP5.v2 puro

   Plasmid

   #87793

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. tet-pLKO shMKP5.v1 puro

   Plasmid

   #87792

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-puro GFP-UVRAG

    Plasmid

    #38267

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    UV radiation resistance-associated gene protein (UVRAG Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pRP(Exp)-CMV> :dTomato:IRES:Puro

    Plasmid

    #110832

    Purpose

    Expresses dTomato (dT) red fluorescent reporter.

    Depositor

    Ande Satyanarayana

    Article

    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

    Insert

    dTomato Tag
    

    Use

    Non-viral gene expression vector

    Tags

    IRES

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSicoR (Puro) p65 shRNA2

    Plasmid

    #22508

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA2 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

13. HA-VHL Y98N-pBabe-puro

    Plasmid

    #19236

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 98 to Asparagin

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

    Plasmid

    #167819

    Purpose

    control sensor for EKAREN5

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5(TA)
    

    Use

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBABE-puro-NLS-LSSmKate1

    Plasmid

    #31873

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate1
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    Available since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. FASTHDR-Cterm-NanoLuc-Puromycin

    Plasmid

    #167209

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. OCT4 GFP puro Donor 2

    Plasmid

    #22210

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlue-loxPuro-APEX-PML_LASA

    Plasmid

    #140273

    Purpose

    a targeting plasmid for APEX-PML

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    APEX2
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    endogenous

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

    Plasmid

    #208554

    Purpose

    Lentiviral vector expressing gRNA targeting human BCL11B-E1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B-E1(14) (BCL11B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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