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pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

Plasmid

#117409

Purpose

SauCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SauCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

Plasmid

#117410

Purpose

LbCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

LbCas12a gRNA targeting TLR 2.0

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113701

Purpose

Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

Plasmid

#113699

Purpose

Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-dTPR

Plasmid

#114048

Purpose

Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-dTPR (TTK Human)

Expression

Mammalian

Mutation

delta TPR (deletion of TPR domain 63-191)

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d200

Plasmid

#114047

Purpose

Expression of exogenous LAP tagged MPS1 delta 200 (NTE and TPR are deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d200 (TTK Human)

Expression

Mammalian

Mutation

delta 200 (deletion of NTE and TPR domains)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d60

Plasmid

#114046

Purpose

Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d60 (TTK Human)

Expression

Mammalian

Mutation

delta 60 (deletion of NTE domain)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP046

Plasmid

#107062

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

Plasmid

#105681

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

Plasmid

#105680

Purpose

Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Synonymous base changes not affecting the protein…

Promoter

tac_Promoter

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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