We narrowed to 9,230 results for: mel
-
Plasmid#80424PurposeUsed for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joiningDepositorInsertSpCas9-Gem
UseCRISPRTagsGemininMutationcodon optimized for humanPromoterT7Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10
Plasmid#162387PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-Bos-FLAG MDA5 MIII
Plasmid#52876PurposeExpresses MDA5 with a mutation to motif III in mammalian cellsDepositorAvailable SinceMay 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10
Plasmid#162384PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8m
Tags6xHisExpressionInsectMutationA25G F286YPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10
Plasmid#162385PurposeLexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
Tags6xHisExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-HD39Q
Plasmid#11514DepositorInserthuntingtin, exon 1, 39 glutamines (HTT Human)
TagsHis, His (out of frame), and TrxExpressionBacterialMutationhuntingtin exon 1 with 39 glutamines, vector m…Available SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_mCh-SspB (pBS1144)
Plasmid#185326PurposeFor the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLB2 5′ mouse CNS
Plasmid#49348Purposeluciferase reporter with mouse Bmp2 promoter and 3' conserved noncoding sequence (CNS)DepositorUseLuciferaseTagsluciferasePromoter-Available SinceDec. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_mCh-SspB (pBS1142)
Plasmid#185324PurposeFor the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE2
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL4 Cluster Promoter Delta 1.2
Plasmid#100130PurposeIt contains 0.5 Kb of the miR-183-96-182 cluster promoter. This fragment partially loses promoter activity in melanoma cells.DepositorInsertmiR-183-96-82 Cluster promoter 0.5 Kb Fragment (MIR182 Human)
UseLuciferaseAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only