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pUdO3c

Plasmid

#210232

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pUdO3s

Plasmid

#210240

Purpose

medium-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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MOBE2

Plasmid

#219942

Purpose

Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

apt-CBE-3'end_PT2A_apt-ABE8.20

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_K262A

Plasmid

#225728

Purpose

To express Mce3R mutant K262A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

K262A

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-MelG

Plasmid

#225729

Purpose

To express MelG (Rv1937) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melG

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pET28b-VapBC35

Plasmid

#225733

Purpose

To express VapBC35(Rv1962) Toxin antitoxin in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

vapBC35

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T_Mce3R

Plasmid

#225726

Purpose

To express Mce3R in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::luxI

Plasmid

#189574

Purpose

Lux synthase (3OC6-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::traI

Plasmid

#189575

Purpose

Tra synthase (3OC8-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBbA2C_Ptet::lasI

Plasmid

#189576

Purpose

Las synthase (3OC12-HSL)

Depositor

Daniel Ducat

Article

Kokarakis et al ACS Synth Biol. 2023 Jan 20;12(1):265-27

Insert

Acyl-homoserine-lactone synthase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pZB604_pT7-sfGFP

Plasmid

#228510

Purpose

Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

sfGFP

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pZB641_pT7_Lysin-KU1

Plasmid

#228511

Purpose

T7 RNAP inducible Lysin KU1 Expression

Depositor

Timothy Whitehead

Article

Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

Insert

Lysin KU1

Use

Synthetic Biology

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxC

Plasmid

#225742

Purpose

To express TrxC(Rv3914) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxC

Tags

6xHis

Expression

Bacterial

Mutation

TrxC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelI

Plasmid

#225730

Purpose

To express MelI (Rv1939) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melI

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelK

Plasmid

#225732

Purpose

To express MelK (Rv1941) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melK

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-MelJ

Plasmid

#225731

Purpose

To express MelJ (Rv1940) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

melJ

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOinT1T2

Plasmid

#225058

Purpose

Heterologous expression in Streptomyces with inactive SnaO

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

inactivated SnaO

Expression

Bacterial

Mutation

deltaR51-V511

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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POSV801-SP44-SnaABCDEOT1T2

Plasmid

#225055

Purpose

Heterologous expression in Streptomyces for threopeptin biosynthetic gene cluster

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Mutation

WT

Promoter

SP44

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-coSnaA-TEV-His_His-TEV-SnaC

Plasmid

#225059

Purpose

recombinant expression of SnaA in E. coli

Depositor

Wilfred van der Donk

Article

Yu et al ACS Cent Sci. 2024 Jun 3;10(6):1242-1250

Insert

SnaA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_LSorbose

Plasmid

#215562

Purpose

Expresses GFP in response to L-Sorbose

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
sorR

Expression

Bacterial

Promoter

P250 and Psorr

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_Isoeugenol

Plasmid

#215563

Purpose

Expresses GFP in response to Isoeugenol

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
iemR

Expression

Bacterial

Promoter

P250 and Piemr

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLigify_4Vinylphenol

Plasmid

#215564

Purpose

Expresses GFP in response to 4-Vinylphenol

Depositor

Simon d'Oelsnitz, David Ross

Article

d'Oelsnitz et al ACS Synth Biol. 2024 Aug 16;13(8):2577-2

Inserts

GFP mut2
vprR

Expression

Bacterial

Promoter

Pvprr

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pUdOm2.2

Plasmid

#210279

Purpose

Empty backbone with ampicillin resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm2.5

Plasmid

#210282

Purpose

Empty backbone with ampicillin resistance for low expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUdOm3.1

Plasmid

#210283

Purpose

Empty backbone with chloramphenicol resistance for high expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge