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pTRKH3-ermGFP

Plasmid

#27169

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

presence of an additional 183-bp region at N term…

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-ldhGFP

Plasmid

#27170

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

lactate dehydrogenase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al bioRxiv 2024.05.31.596797

Insert

PLIN3 (PLIN3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTRKH3-slpGFP

Plasmid

#27168

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pCCl-MCS_miniCMV_EGFP

Plasmid

#134984

Purpose

The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

Depositor

Claudio Mussolino

Article

Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

minCMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pNIC-CT-sfGFP-HN-GG

Plasmid

#215803

Purpose

Cloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tag

Depositor

Frank von Delft, ASAP Discovery

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJet1.2 sfGFP

Plasmid

#117123

Purpose

C-terminal GFP tag

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Insert

Superfolder GFP

Use

Golden gate donor vector for gene targeting in ba…

Tags

sfGFP

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pENV8(WT)-GFPuv

Plasmid

#70127

Purpose

Cobalamin riboswitch reporter vector for use in E. coli

Depositor

Robert Batey

Article

Johnson JE et al Nature. 2012 Dec 6;492(7427):133-7. doi:

Insert

wild type environmental cobalamin riboswitch sequence (env8)

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-slpGFP

Plasmid

#27171

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-ermGFP

Plasmid

#27172

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pPro24(s)-gfp

Plasmid

#17813

Depositor

Jay Keasling

Article

Lee et al Biotechnol Bioeng. 2006 Apr 5. 93(5):912

Insert

GFPuv

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

D234N compared to AF007834

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pUC19/DdmEGFP

Plasmid

#154304

Purpose

cloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-PP-GFP

Plasmid

#135390

Purpose

Expresses OSCA1.2 in mammalian cells

Depositor

Ardem Patapoutian

Article

Jojoa-Cruz et al Elife. 2018 Nov 1;7. pii: 41845. doi: 10

Insert

OSCA1.2 (AT4G22120 Mustard Weed)

Use

Tags

EGFP tag connected to the gene via a precision pr…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBW414hrpL-cIgfp

Plasmid

#61439

Purpose

pSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs34-CI-LVA-t-plam-30gfp-t

Use

Tags

LVA

Expression

Bacterial

Mutation

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pmCD8-GFP,y+

Plasmid

#24350

Depositor

Liqun Luo

Article

Potter et al Volume 141, Issue 3, 536-548, 30 April 2

Insert

Yellow gene

Use

Tags

CD8-GFP

Expression

Insect

Mutation

Promoter

Available since

April 12, 2010

Availability

Academic Institutions and Nonprofits only

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pBW412hrpL-cIgfp

Plasmid

#61438

Purpose

pSB4A3 harbouring hrpL-rbs32-cI-lva-ter-Plam-rbs30-gfp-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

HrpL-rbs32-CI-LVA-t-plam-30gfp-t

Use

Tags

LVA

Expression

Bacterial

Mutation

Promoter

HrpL

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP

Plasmid

#48988

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_AAN_4

Plasmid

#231415

Purpose

This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-X
sgRNA-Y

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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LI D-E TurboID-GFP

Plasmid

#209387

Purpose

Golden Gate LI D-E module

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Type

Empty backbone

Use

Cloning vector

Tags

Expression

Mutation

TurboID gene sequence C249A

Promoter

Available since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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