We narrowed to 2,540 results for: organization Addgene

1. Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

   Plasmid

   #168772

   Purpose

   Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   Hrasls (plaat1 Zebrafish)
   

   Tags

   EGFP

   Promoter

   alphaA-crystallin promoter

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP_V577D-talin1 (1-2541)

   Plasmid

   #166115

   Purpose

   expresses talin-1-V577D in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   V577D-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP_P229L-talin1 (1-2541)

   Plasmid

   #166113

   Purpose

   expresses talin-1-P229L in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

   Insert

   P229L-Talin-1 (Tln1 Mouse)
   

   Use

   Cmv promoter

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA-DAB1

   Plasmid

   #162691

   Purpose

   Expresses DabAB1 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB1 inorganic carbon transport complex
   

   Expression

   Bacterial

   Promoter

   PLtet0-1 promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFE-dabAB2 C539A

   Plasmid

   #133008

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   C539A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFE-dabAB2 H524A

   Plasmid

   #133007

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   H524A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFE-dabAB2 D353A

   Plasmid

   #133006

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxIS

   Plasmid

   #131354

   Purpose

   Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   Caspase-1 (C285A)-NpLxIS (Casp1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Caspase-1 (C285A)

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-HT_SsCHAD-H29A-R32A-R36A

   Plasmid

   #127786

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32 and Arg36 to Ala

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. OMMGFP 1-10

    Plasmid

    #164119

    Purpose

    Expresses the non-fluorescent GFP1-10 moiety on the cytosolic face of the OMM

    Depositor

    Tito Calì, Marisa Brini

    Article

    Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

    Insert

    OMMGFP 1-10
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPLICS Mt-ER- PM Short P2A

    Plasmid

    #164110

    Purpose

    Detect the short Plasma membrane-mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM- Mt-ER Short P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SPLICS Mt-ER- PM Long P2A

    Plasmid

    #164109

    Purpose

    Detect the long Plasma membrane--mitochondria-Endoplasmic reticulum contact

    Depositor

    Tito Calì, Marisa Brini

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split GFP and YFP PM-Mt-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. FAB(ER-mito)

    Plasmid

    #214427

    Purpose

    Detection of ER-mito contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST10-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-TetO-PM

    Plasmid

    #197387

    Purpose

    To establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    PDX1-T2A-MAFA
    

    Use

    Lentiviral; Teto

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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