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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1841 - 1860 of 13578 results

  10. pJCC_164 SpCas9 R1333A/R1335A

    Plasmid
    #179528
    Purpose
    For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 R1333A/R1335A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed arginines 1333 and 1335 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179528-plasmid-map-sequence-id-351744

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  18. NmMetQ

    Plasmid
    #172111
    Purpose
    Expression of Methionine Binding Protein (MetQ) with signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine
    Promoter
    T7 Promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172111-plasmid-map-sequence-id-344824

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  19. pBS-V7

    Plasmid
    #173796
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene latifolia clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Expression
    Bacterial and Plant
    Available Since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173796-plasmid-map-sequence-id-342770

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  20. pBS-V6

    Plasmid
    #173795
    Purpose
    pBS plasmid containing aadA gene with 16S rRNA promoter and psbA 3’ region, Silene conica clpP1 gene with the tobacco regulatory elements and flanking regions for tobacco plastome transformation
    Depositor
    Daniel Sloan
    Article
    Abdel-Ghany et al Plant Mol Biol. 2022 Feb;108(3):277-287.
    Inserts
    aadA
    ClpP1
    Expression
    Bacterial and Plant
    Available Since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    173795-plasmid-map-sequence-id-342769

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Showing: 1841 - 1860 of 13578 results