159,481 results

1. pMJ114

   Plasmid

   #85995

   Purpose

   One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified bovine U6-2

   Available since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2111

   Plasmid

   #141928

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FgH1tUTG_mup53_Ex5

   Plasmid

   #85535

   Purpose

   Inducible expression of guide RNA (mup53_Ex5) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   mu p53
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1t

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBG100-TCPTP

   Plasmid

   #33365

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   TCPTP-45 (PTPN2 Human)
   

   Use

   Tags

   6xHIS - cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pYTK066

   Plasmid

   #65173

   Purpose

   Encodes tTDH1 as a Type 4b part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   tTDH1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-HA-CUL1 delta N53

   Plasmid

   #19950

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   cullin 1 delta N53 (CUL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deletion of 53 residues from the NH2-terminus(de…

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pU6a:sgRNA#2

   Plasmid

   #64246

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFL-SV40-p38 β/MAPK11 5'-UTR

   Plasmid

   #115357

   Purpose

   firefly luciferase (Fluc) reporter containing the 5’-UTR of p38 β/MAPK11 mRNA

   Depositor

   Ming-Chih Lai

   Article

   Ku et al Mol Cell Biol. 2018 Dec 11;39(1). pii: M

   Insert

   p38 β/MAPK11 5'-UTR (MAPK11 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD02_CBASS_Escherichia_coli_SLK172

   Plasmid

   #224395

   Purpose

   For expression of the type I CBASS system encoded by Escherichia coli SLK172

   Depositor

   Philip Kranzusch

   Article

   Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

   Insert

   EcCdnD_4TM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-HA-CUL1 delta C22

    Plasmid

    #19942

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    cullin 1 delta C22 (CUL1 Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Mutation of 22 amino acids from the C terminus

    Promoter

    Available since

    April 17, 2009

    Availability

    Academic Institutions and Nonprofits only