Skip to main content
Addgene
Showing: 3721 - 3760 of 8321 results

  1. FLAG-hMYOD1 (#794)

    Plasmid
    #78329
    Purpose
    Overexpression of human MYOD1 ORF with a N-terminal FLAG tag
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  2. pAP84

    Plasmid
    #86554
    Purpose
    Expresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficile
    Depositor
    Wiep Klaas Smits
    Insert
    Pveg-sLucopt
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86554-plasmid-map-sequence-id-173277

    Enlarge

  3. pcDNA-Dlx6

    Plasmid
    #99480
    Purpose
    Mammalian expression of the Distal-less Homeobox 6 gene
    Depositor
    Jhumku Kohtz
    Insert
    Dlx6 (Dlx6 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    99480-plasmid-map-sequence-id-190484

    Enlarge

  4. pSBBi-Pur LaG17-synNotch-TetRVP64

    Plasmid
    #158133
    Purpose
    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone
    Depositor
    Neal Devaraj
    Insert
    LaG17-synNotch-TetRVP64
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    158133-plasmid-map-sequence-id-309383

    Enlarge

  5. pSBbi-Pur/GDI1-splitTurboID

    Plasmid
    #198219
    Purpose
    Transposon vector (Addgene #60523) for expression of part of TurboID (up to E256) and GID1, for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    GID1
    Use
    Transposon
    Tags
    5’ part of TurboID, up to E256
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198219-plasmid-map-sequence-id-389399

    Enlarge

  6. ER-SPLICSs-cFAST10

    Plasmid
    #210431
    Purpose
    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    ER-SPLICSs-cFAST10
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210431-plasmid-map-sequence-id-415626

    Enlarge

  7. SLC9A3R2-TwinStrepII

    Plasmid
    #83200
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    SLC9A3R2 (Slc9a3r2 Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83200-plasmid-map-sequence-id-162200

    Enlarge

  8. pCMV-Neo-Bam E2F3b

    Plasmid
    #37975
    Depositor
    Jacqueline Lees
    Insert
    E2F3b (E2F3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3.1-Letm1

    Plasmid
    #191962
    Purpose
    Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).
    Depositor
    Luca Scorrano
    Insert
    Letm1 (LETM1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191962-plasmid-map-sequence-id-379658

    Enlarge

  10. SEPX1

    Plasmid
    #39048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEPX1 (MSRB1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys95-S
    Promoter
    Available since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Jesse Rinehart
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetR/pLtetO
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52053-plasmid-map-sequence-id-442992

    Enlarge

  12. YWHAQ

    Plasmid
    #38931
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    YWHAQ (YWHAQ Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pLV V5-lamin B2

    Plasmid
    #175109
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb2
    Depositor
    Larry Gerace
    Insert
    Lmnb2 (Lmnb2 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    175109-plasmid-map-sequence-id-351741

    Enlarge

  15. pSMH1741

    Plasmid
    #160930
    Purpose
    Expression of soluble Sc octamer (H3)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    HHT1
    Use
    Tags
    6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160930-plasmid-map-sequence-id-318962

    Enlarge

  16. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

    Plasmid
    #121542
    Purpose
    Chronos with red nucleus-targeted fluorescence for excitatory neurons
    Depositor
    Matthias Prigge
    Insert
    blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    CamKII
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121542-plasmid-map-sequence-id-308124

    Enlarge

  17. pDONR221-TMPRSS4

    Plasmid
    #158446
    Purpose
    Gateway Cloning compatible entry vector for the human TMPRSS4 gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    TMPRSS4 (TMPRSS4 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    158446-plasmid-map-sequence-id-308254

    Enlarge

  18. pSMH1742

    Plasmid
    #160929
    Purpose
    Expression of soluble Sc octamer (Cse4)
    Depositor
    Stephen Harrison
    Inserts
    HHF1
    HTA1
    HTB1
    Cse4
    Use
    Tags
    6His-TEV, N-terminal 6His-TEV, and none
    Expression
    Mutation
    codon-optimized for E. coli -- see provided seque…
    Promoter
    Available since
    Dec. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    160929-plasmid-map-sequence-id-319094

    Enlarge

  19. pE-SUMO CRYAA

    Plasmid
    #80756
    Purpose
    Expression of human alpha A-crystallin in E. coli
    Depositor
    Larry David
    Insert
    CRYAA (CRYAA Human)
    Use
    Tags
    6xHIS and Smt3 SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    80756-plasmid-map-sequence-id-153571

    Enlarge

  20. AAV-CAG-K-SPOTIT

    Plasmid
    #191488
    Purpose
    Green fluorescent-based opioid sensor for the kappa opioid receptor in AAV viral vector under a CAG promoter
    Depositor
    Wenjing Wang
    Insert
    K-SPOTIT
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191488-plasmid-map-sequence-id-377866

    Enlarge

  21. AXL pBABE

    Plasmid
    #105936
    Purpose
    retroviral expression of AXL
    Depositor
    Rosa Marina Melillo
    Insert
    AXL (AXL Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    105936-plasmid-map-sequence-id-204362

    Enlarge

  22. EF1α-eGFP-Z1

    Plasmid
    #135612
    Purpose
    Enhanced green fluorescent protein expression for mammalian cells under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135612-plasmid-map-sequence-id-269403

    Enlarge

  23. Rhesus AAVS1-CAG-copGFP

    Plasmid
    #84209
    Purpose
    Rhesus AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP
    Depositor
    Cynthia Dunbar
    Inserts
    Rhesus AAVS1 5’ homology arm
    Rhesus AAVS1 3’ homology arm
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    84209-plasmid-map-sequence-id-166261

    Enlarge

  24. pBV514

    Plasmid
    #204602
    Purpose
    Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4335696 Oryza sativa)
    Use
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Mutation
    Changed phenylalanine 74 into an alanine (F74A).
    Promoter
    ScADH1
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204602-plasmid-map-sequence-id-417406

    Enlarge

  25. p5E-mitfa2.1

    Plasmid
    #81234
    Purpose
    zebrafish mitfa promoter entry vector for multisite Gateway cloning
    Depositor
    James Lister
    Insert
    mitfa promoter (mitfa Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    mitfa, 2.1kb including 5' UTR
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    81234-plasmid-map-sequence-id-157888

    Enlarge

  26. IRES-creERT2-FNF

    Plasmid
    #188230
    Purpose
    EMCV IRES-cre-ERT2-FRT-Neo*-FRT cassette
    Depositor
    Mario Capecchi
    Insert
    IRES-creERT2-FNF
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    188230-plasmid-map-sequence-id-371215

    Enlarge

  27. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid
    #110375
    Purpose
    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    turboGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110375-plasmid-map-sequence-id-213401

    Enlarge

  28. RACGAP1

    Plasmid
    #38807
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RACGAP1 (Racgap1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. PsbA2-HMGS-HMGR-ATOB-NPTI

    Plasmid
    #73338
    Purpose
    Construct expressing the upper MVA pathway in Synechocystis PCC 6803
    Depositor
    Anastasios Melis
    Insert
    HMGS-HMGR-ATOB-NPTI
    Use
    Tags
    Expression
    Mutation
    Promoter
    psbA2
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    73338-plasmid-map-sequence-id-135049

    Enlarge

  30. PIM2

    Plasmid
    #38867
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIM2 (PIM2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. 7M3X

    Plasmid
    #176411
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    RBBP7 (RBBP7 Human)
    Use
    Baculovirus expresssion
    Tags
    6xHis-TEV
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176411-plasmid-map-sequence-id-345851

    Enlarge

  32. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

    Enlarge

  33. CAG-eGFP-Z1

    Plasmid
    #135046
    Purpose
    Enhanced Green fluorescent protein under constitutive CAG Promoter for mammalian expression in minimal backbone plasmid
    Depositor
    Jordan Green
    Insert
    eGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135046-plasmid-map-sequence-id-269409

    Enlarge

  34. pRFP.mIKKε

    Plasmid
    #74414
    Purpose
    Expresses mouse RFP-IKKε in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    mouse IKKε (Ikbke Mouse)
    Use
    Tags
    RFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74414-plasmid-map-sequence-id-140322

    Enlarge

  35. CMV-AausFP1-Z1

    Plasmid
    #135048
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under CMV promoter in minimal backbone plasmid.
    Depositor
    Jordan Green
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    135048-plasmid-map-sequence-id-269407

    Enlarge

  36. pCDZ2FE_Pogz_1-1410

    Plasmid
    #105674
    Purpose
    Expresses Flag-tagged full-length human POGZ protein
    Depositor
    Thanos Halazonetis
    Insert
    POGZ (POGZ Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105674-plasmid-map-sequence-id-203198

    Enlarge

  37. pFLAG-6a-Ataxin3Q22

    Plasmid
    #22126
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2009
    Availability
    Academic Institutions and Nonprofits only

  38. pLenti6.3_DTX1

    Plasmid
    #124080
    Purpose
    DTX1 with normal stop codon, 3 silent SNPs (1233) C>T, (1251) A>G, (1425) C>T)
    Depositor
    J. Chad Brenner
    Insert
    DTX1 (DTX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124080-plasmid-map-sequence-id-241731

    Enlarge

  39. mCherry-CenpA-N-22

    Plasmid
    #62742
    Purpose
    Localization: Nucleus, N1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  40. pT7CFE1_polio_3ABC

    Plasmid
    #212986
    Purpose
    Encodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus Type I Mahoney 3ABC
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212986-plasmid-map-sequence-id-420355

    Enlarge

Showing: 3721 - 3760 of 8321 results