Addgene

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pZS039

Plasmid

#134233

Purpose

For in vitro transcription of Miniata CENP-A

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Miniata CENP-A n-terminus (nt 1-204, aa 1-68)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-EMTB-GFP-ePDZ

Plasmid

#135600

Purpose

Expression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

EMTB-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP-ePDZ

Plasmid

#135602

Purpose

Expression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_HsPDZ1b

Plasmid

#131216

Purpose

Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

HsPDZ1b

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_HsPDZ1b_X

Plasmid

#131214

Purpose

Expresses HsPDZ1b (Homo sapiens) folllwed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

HsPDZ1b

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_X_AtPIF6

Plasmid

#131192

Purpose

Expresses AtPIF6 (Arabidopsis thaliana) preceded by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPIF6

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10-SNAP

Plasmid

#131161

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10-SNAP

Use

Bicistronic

Tags

Cdc10: SNAP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSM002

Plasmid

#122936

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML043C (Marked)

Use

Tags

Expression

Yeast

Mutation

YML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATAC

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSM001

Plasmid

#122935

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEC005

Plasmid

#122939

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML071C (Marked)

Use

Tags

Expression

Yeast

Mutation

YML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGC

Promoter

[tetO2]4inPgal1

Available since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_207

Plasmid

#122938

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML070W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTA

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_243

Plasmid

#122940

Purpose

Expression of the marked mRNA under the control of insertional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML029W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTA

Promoter

Pyml029w-tetO(2)

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSM004

Plasmid

#122937

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YML065W (Marked)

Use

Tags

Expression

Yeast

Mutation

YML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAAC

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_228

Plasmid

#122933

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR025C (Marked)

Use

Tags

Expression

Yeast

Mutation

YBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTA

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_229

Plasmid

#122934

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR026C (Marked)

Use

Tags

Expression

Yeast

Mutation

YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS415

Plasmid

#114272

Purpose

SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

Depositor

Erik Jorgensen

Article

Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

Insert

Cbr-Unc-119

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

*please see depositor comment below

Promoter

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0114

Plasmid

#117553

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana

Use

Tags

Expression

Plant

Mutation

Promoter

At U6-26 (pICSL90002)

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0029

Plasmid

#117541

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (LbCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0028

Plasmid

#117540

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

crRNA (AsCas12a)

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0014

Plasmid

#117524

Purpose

Level0 Golden Gate part: CDS, SpCas9-KA

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-KA (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

K855A

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0009

Plasmid

#117522

Purpose

Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-p (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL90005

Plasmid

#117520

Purpose

Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SpCas9-h (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0008

Plasmid

#117535

Purpose

Level0 Golden Gate part: CDS, AsCas12a

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

AsCas12a (with stop codon)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mutation

Promoter

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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p005-RFP-weak

Plasmid

#108316

Purpose

Synthetic RFP based on Corynactis spp under weak synthetic constitutive promoter pFAB4024; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

RFP under controlf of weak synthetic constitutive promoter pFAB4024

Use

Tags

Expression

Bacterial

Mutation

Promoter

pFAB4024

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Tags

Expression

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Use

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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env8 16nt J1/13

Plasmid

#99832

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 16nt J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 16 nucleotides

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
env8 19nt J1/13

Plasmid

#99834

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 19nt J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 19 nucleotides

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 17nt J1/13

Plasmid

#99833

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 17nt J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 17 nucleotides

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 24nt J1/13

Plasmid

#99835

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 24nt J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 24 nucleotides

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
env8 7U J1/13

Plasmid

#99829

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 7U J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to 7 U's

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
env8 reverse J1/13

Plasmid

#99831

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 reverse J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 sequence is reversed

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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env8 AC rep J1/13

Plasmid

#99830

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 AC rep J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 converted to repeating AC's

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
env8 25nt J1/13

Plasmid

#99836

Purpose

GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

Depositor

Robert Batey

Article

Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

Insert

env8 25nt J1/13

Use

Tags

GFPuv

Expression

Bacterial

Mutation

J1/13 with 25 nucleotides

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-MAVS-M1A(KaganE18)

Plasmid

#52009

Purpose

Expresses the human MAVS CDS containing the point mutation M1A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala

Promoter

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M142A(KaganE19)

Plasmid

#52010

Purpose

Expresses the human MAVS CDS containing the point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pAGM5355

Plasmid

#50296

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic) + HIS tag

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM1467

Plasmid

#50298

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + HIS tag + enterokinase cleavage site

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH88103

Plasmid

#50273

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, ocs, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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