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pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Burden Monitor phi80 version

Plasmid

#66074

Purpose

The burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

GFP

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pES-PmeI

Plasmid

#122698

Purpose

To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Type

Empty backbone

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pNSA-ARS-CRISPR-BlastR

Plasmid

#101009

Purpose

Expresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMBA334

Plasmid

#214746

Purpose

High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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gRNAy

Plasmid

#168294

Purpose

Expresses gRNA targeting the yellow gene in Drosophila

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

U6.3-gRNA[y]-scaffold

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM51559

Plasmid

#153226

Purpose

Control plasmid for knockout of TRY and CPC genes

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Cas9, guide RNA for AP3, FAST marker

Use

Synthetic Biology

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-sfGFP-3xP3-RFP

Plasmid

#127557

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Expression

Insect

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-intron

Plasmid

#177804

Purpose

An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

intron

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P33

Plasmid

#120018

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP2

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TrpLE-M-nMSP1E3D1

Plasmid

#179949

Purpose

Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

Depositor

Kerney Glover

Article

Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

Insert

TrpLE-M-nMSP1E3D1

Tags

TrpLE

Expression

Bacterial

Mutation

Native Trp residues have been mutated to Phe resi…

Promoter

T7

Available Since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMBA328

Plasmid

#214748

Purpose

Medium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RBS(22821)-mcherry

Expression

Bacterial

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA333

Plasmid

#214745

Purpose

Medium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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