We narrowed to 2,317 results for: crispr system

1. PiggyBac dCas9-VPR

   Plasmid

   #191269

   Purpose

   piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   VPR-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PiggyBac dCas9-KRAB

   Plasmid

   #191314

   Purpose

   piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

   Depositor

   Joanna Wysocka

   Article

   Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

   Inserts

   KRAB-dCas9-HA-tagBFP
   rtTA3-IRES-PURO
   

   Use

   CRISPR

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYTK050

   Plasmid

   #65157

   Purpose

   Encodes sgRNA Dropout as a Type 234 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

   Plasmid

   #192147

   Purpose

   T cell CRISPR lentiviral vector

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

   Plasmid

   #92099

   Purpose

   Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR and TALEN; Zfn

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-On 3G Bidirectional

   Available since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1alpha

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCW-tTRE-scFv-SunTag

   Plasmid

   #193138

   Purpose

   Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

   Depositor

   Brad Rosenberg

   Article

   Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

   Insert

   scFv-SunTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330_human CDK1

   Plasmid

   #118597

   Purpose

   One shot system to create CDK1as human cells. Encode Cas9 and guideRNA to inactivate human CDK1 gene.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   guide RNA against human CDK1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

   Plasmid

   #84247

   Purpose

   Expresses direct fusion VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, VPR, and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-Csy4-PE2

    Plasmid

    #172667

    Purpose

    Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

    Insert

    Csy4-PE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid

    #84246

    Purpose

    Expresses direct fusion VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sa dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pL-SFFV.Reporter.RFP657.PAC

    Plasmid

    #61395

    Purpose

    Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMT1-24X-NOG(CmYLCV)

    Plasmid

    #202014

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Use

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUPD2 AtUBQ10 (GB2478)

    Plasmid

    #160594

    Purpose

    UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    AtUBQ10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

    Plasmid

    #192148

    Purpose

    T cell CRISPR AAV vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-PGK-scFv GCN4-sfGFP-VP64

    Plasmid

    #122133

    Purpose

    Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    scFv GCN4-sfGFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    RRM2 endogenous promoter

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-U6-sgRNA_VEGFA-mcherry

    Plasmid

    #159790

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting VEGFA gene (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge