We narrowed to 14,886 results for: pET

1. pET28a-eGFP-MinD

   Plasmid

   #133622

   Purpose

   Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

   Depositor

   Petra Schwille

   Article

   Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

   Insert

   EGFP-MinD (minD E. coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_mouse-pro-IL1B

   Plasmid

   #183386

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL1B (Il1b Mouse)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21a Ce-iPGM-bio

   Plasmid

   #162559

   Purpose

   Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   C. elegans iPGM bio-6His (ipgm-1 Nematode)
   

   Use

   Tags

   6His tag, T7 tag, Thrombin cleavage site, and bio…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a Bm-iPGM-bio

   Plasmid

   #162565

   Purpose

   Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

   Depositor

   James Inglese

   Article

   Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

   Insert

   B. malayi iPGM bio-6His
   

   Use

   Tags

   6His tag, T7 tag, biotinylation sequence, and thr…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-29b(+)-mSandy2

   Plasmid

   #177760

   Purpose

   Expresses mSandy2 using the T7 Promoter

   Depositor

   Roberto Chica

   Article

   Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

   Insert

   mSandy2
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-C2m2-SNAP

   Plasmid

   #208773

   Purpose

   To express C2m2-SNAP in bacteria

   Depositor

   Urte Neniskyte

   Article

   Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

   Insert

   C2m2-SNAP (Mfge8 Mouse)
   

   Use

   Tags

   SNAP-tag

   Expression

   Bacterial

   Mutation

   Mutated 24 and 45 lysine to asparagine in C2 doma…

   Promoter

   T7 promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6his-WT gusA-pET28a+

   Plasmid

   #61156

   Purpose

   N -terminal His tagged wild-type GUS expression vector

   Depositor

   Ichiro Matsumura, Andrew Ellington

   Article

   Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

   Insert

   gusA
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-HIS-Tol2

    Plasmid

    #112486

    Purpose

    functional recombinant Tol2 protein from E.coli

    Depositor

    Stephen Ekker

    Article

    Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

    Insert

    Tol2 Transposase (tpase Synthetic)
    

    Use

    Tags

    HIS/6x

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11a-link-NGFP

    Plasmid

    #52732

    Purpose

    IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-NGFP
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-HisYFP-Sp100

    Plasmid

    #53141

    Purpose

    E.coli expression of HisYFP tagged human Sp100 fragment

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    Sp100A fragment (SP100 Human)
    

    Use

    Tags

    His-YFP

    Expression

    Bacterial

    Mutation

    fragment (amino acids 71-480); deletion of L75

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-11-LECT2-I40

    Plasmid

    #183032

    Purpose

    Expression of LECT2 I40 variant protein from T7 promoter

    Depositor

    Gareth Morgan

    Article

    Belonogov et al bioRxiv 2022.03.01.482540

    Insert

    LECT2 (LECT2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I40 variant

    Promoter

    T7

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28C-mCherry-HNRNPA1LCD

    Plasmid

    #204405

    Purpose

    E. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    HNRNPA1 (186-320) (HNRNPA1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-miniE-DBCO

    Plasmid

    #181985

    Purpose

    Used to label proteins with the MiniR genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniE-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28C-mCherry-DDX4LCD

    Plasmid

    #204408

    Purpose

    E. coli expression of DDX4 low-complexity domain (1-236) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    DDX4(1-236) (DDX4 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-15b-PTS

    Plasmid

    #108953

    Purpose

    heterologous expression of PTS of P. cablin in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    Patchoulol synthase (PTS)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-APOBEC1-YTH

    Plasmid

    #200158

    Purpose

    For bacterial expression of the APOBEC1-YTH fusion protein for protein purification

    Depositor

    Kate Meyer

    Article

    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

    Insert

    APOBEC1-YTH
    

    Use

    Tags

    6xHis, HA, and Maltose Binding Protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E coli expression

    Promoter

    LacZ

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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