We narrowed to 9,921 results for: pET

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pEE065

Plasmid

#176822

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

704

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEE050

Plasmid

#176807

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

601

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-link-ymYPET-URA3

Plasmid

#168056

Purpose

Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Tags

ymYPET

Expression

Yeast

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-6His-SUMO-Nsp10

Plasmid

#154959

Purpose

Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pet28a-His6-BS2-TRFLE

Plasmid

#247993

Purpose

BS2-TRFLE bacterial expression plasmid

Depositor

Jeffrey Martell

Publication

Ogorek et al ACS Cent. Sci. 2026,

Insert

BS2-TRFLE Esterase

Tags

His Tag

Expression

Bacterial

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-SpyCatcher3

Plasmid

#235149

Purpose

Bacterial expression of His-tagged SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Publication

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b 6His SpyCatcher-GRFT

Plasmid

#204502

Purpose

Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

Depositor

Urartu Seker

Publication

Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

Insert

GRFT

Use

Synthetic Biology

Tags

6xHis and SpyCatcher

Expression

Bacterial

Promoter

T7

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-his6-HA3-CLIP

Plasmid

#188942

Purpose

T7 expression vector for making his6- and HA3-tagged CLIP protein

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

his6-HA3-CLIP

Expression

Bacterial

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [282-490]

Plasmid

#186469

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Publication

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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His-SUMO-flag-hDDI2_pET11a

Plasmid

#169121

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-TAX-L10-STEPtag

Plasmid

#159375

Purpose

Expresses STEPtag fused to the C-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Publication

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

TAX-L10-STEPtag

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag-L10-TAX

Plasmid

#159374

Purpose

Expresses STEPtag fused to the N-terminus of inactive T. aurantiacus xylanase 10A, as a mock protein of interest.

Depositor

Roberto Chica

Publication

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag-L10-TAX

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET11d-FHis-DND1-Y102A

Plasmid

#70082

Purpose

Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

Depositor

Thomas Tuschl

Publication

Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

Insert

Dnd1 (DND1 Human)

Tags

6xHIS and FLAG

Expression

Bacterial

Mutation

Y102A. Codon optimized for bacterial expression

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEThT-MAHS delta MTP

Plasmid

#90032

Purpose

To express His-tagged MAHS in bacteria

Depositor

Takekazu Kunieda

Publication

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Tags

His

Expression

Bacterial

Mutation

Deletion of mitochondria targeting peptide

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Publication

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12-129/138

Plasmid

#47926

Purpose

Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

Depositor

Tanja Kortemme

Publication

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis and TEV Cleavage

Expression

Bacterial

Mutation

Mutations C9A, K129C, D138C

Promoter

T7

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b/EYFP-GgVcl 1-258

Plasmid

#46263

Depositor

Susan Craig

Publication

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-258 (VCL Chicken)

Tags

EYFP and His

Expression

Bacterial

Mutation

aa 1-258 (VD1)

Promoter

T7

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pET24a-LaM4-EGFP

Plasmid

#182641

Purpose

Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, EGFP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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