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pcDNA3-taiman

Plasmid

#17584

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pcold-6xHis-HRV3Csite-HsPACT

Plasmid

#41097

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

PACT (PRKRA Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pCMV2-OCT-HA-eGFP

Plasmid

#67481

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Expression

Mammalian

Promoter

attenuated CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pANY1

Plasmid

#110949

Purpose

Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

Depositor

Yingfeng An

Article

Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

Insert

ccd B cassette

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pattB-3xFlag-Loqs-PB

Plasmid

#41109

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PB (loqs Fly)

Tags

3xFlag

Expression

Insect

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-eBE-S1

Plasmid

#101092

Purpose

Expresses eBE-S1 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-hs-taiman

Plasmid

#17585

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Expression

Insect

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pGTPA-lb

Plasmid

#171528

Purpose

Plasmid contains the Cas12k gRNA for mutant library construction

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGTPA

Plasmid

#171525

Purpose

Plasmid contains the Cas12k gRNA and transposon donor cassette

Depositor

Quanjiang Ji

Article

Chen et al Cell Rep. 2021 Aug 31;36(9):109635. doi:

Insert

Cas12k gRNA, transposon donor cassette

Use

CRISPR

Promoter

Constitutive promoter J23119

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Salsa-BC268-P3G4

Plasmid

#203109

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC268

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC268

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1B

Plasmid

#198335

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1B

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-AP-1 σ1A

Plasmid

#198334

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AP-1 σ1A

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Expresses a LELQMNRRY nonapeptide after the last …

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GB3412

Plasmid

#193109

Purpose

Minimal promoter of NtPCPS2 gene containing 62bp upstream the transcription start site and the 5'UTR region (from GB1027 sequence).

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minNtPCPS2

Use

Synthetic Biology

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

MycHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pVD2

Plasmid

#165857

Purpose

universal Vector Domesticator vector v2 used in the integration (domestication) of new vector backbone parts in the GB2.0 cloning system. Uses blue-white screening method

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL4AD 768-881

Plasmid

#165820

Purpose

768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

GAL4AD 768-881

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Promoterless TP10

Plasmid

#165787

Purpose

Drosphila TP10 poly(a) terminator sequence in promoter grammar, for assembly of no expression control vectors

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Promoterless T10

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#1

Plasmid

#171827

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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