Addgene

Showing: 1881 - 1900 of 4082 results

Results per page:

Sort results by:

pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

Plasmid

#196086

Purpose

Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting lncRNA NORAD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3

Plasmid

#170177

Purpose

Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2s-ST-P2A-NLS-mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pDK637

Plasmid

#134203

Purpose

bacterial expression of human CENP-N 1-240

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-N 1-240-His (CENPN Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX APP delta CT

Plasmid

#30143

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein (APP Human)

Use

Tags

Expression

Mammalian

Mutation

deletion of C-terminal intracellular domain (afte…

Promoter

Available since

July 29, 2011

Availability

Academic Institutions and Nonprofits only
pCMV3Tag8li-hMBOAT7-3xFLAG

Plasmid

#165474

Purpose

Human MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Membrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

Plasmid

#108735

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAX APPs-695-alpha

Plasmid

#30147

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

Amyloid Precursor Protein - soluble (APP Human)

Use

Tags

Expression

Mammalian

Mutation

amino acids 1–612

Promoter

Available since

Aug. 15, 2011

Availability

Academic Institutions and Nonprofits only
pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA

Plasmid

#108789

Purpose

Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment N-terminus to aa233

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA

Plasmid

#108790

Purpose

Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa234 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108770

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

Plasmid

#170175

Purpose

Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-ST-P2A-NLS-mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA

Plasmid

#108743

Purpose

Expresses N-terminus to aa256 of Cre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa256

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA

Plasmid

#108744

Purpose

Expresses aa257 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa257 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER12 (miR-RUL)

Plasmid

#46935

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA

Plasmid

#108771

Purpose

Expresses N-terminus to aa172 of VCre recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment N-terminus to aa172

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA

Plasmid

#108772

Purpose

Expresses aa173 to C-terminus of VCre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

VCre recombinase fragment aa173 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-hFADD-FLAG

Plasmid

#31814

Depositor

Astar Winoto

Article

Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

Insert

FADD (FADD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_Xph15-GCaMP6f

Plasmid

#135538

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to GCaMP6f

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

Tags

GCaMP6f

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA

Plasmid

#108816

Purpose

Expresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Bxb1 integrase fragment aa469 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge