We narrowed to 5,049 results for: golden gate
-
Plasmid#239920PurposesgRNA compatible with TCTP-SynPro-06, TCTP-SynPro-13 and CaMV 35S-SynPro-10 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1000
Plasmid#239922PurposesgRNA compatible with TCTP-SynPro-08 and CaMV 35S-SynPro-04 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1007
Plasmid#239929PurposesgRNA compatible with TCTP-SynPro-04, TCTP-SynPro-11 and CaMV 35S-SynPro-08 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1008
Plasmid#239930PurposesgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
LLP1009
Plasmid#239931PurposesgRNA compatible with TCTP-SynPro-10 as Level-0 part (Golden Gate cloning)DepositorInsertsgRNA
UseSynthetic BiologyMutationN/AAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF_AaRaf1/Raf2/RbcX2/BSD2
Plasmid#229507PurposeExpresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2DepositorInsertsRubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
BSD2
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF_AaRaf1/Raf2/RbcX1/BSD2
Plasmid#229508PurposeExpresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX1,BSD2DepositorInsertsRubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX1
BSD2
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPSmF
Plasmid#226912PurposeMinimal plasmid for Plant STARR-seq with enhancer candidates in forward orientationDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPSmR
Plasmid#226913PurposeMinimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientationDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235301PurposeComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2-bGH
Plasmid#235300PurposeComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH
Plasmid#235302PurposeComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)DepositorInsertFXN-P2A-mRuby2 (FXN Human)
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP-FMRP-bGH
Plasmid#235265PurposeComMAND EGFP-FMRP base geneDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH
Plasmid#235266PurposeComMAND EGFP-FMRP open-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH
Plasmid#235267PurposeComMAND EGFP-FMRP closed-loop circuitDepositorInsertEGFP-Fmr1 (Fmr1 Mouse)
UseSynthetic BiologyTagsFLAGExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234370PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-13X-NOG(AtUBI10)-MTAP-LUC
Plasmid#234371PurposeT-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)DepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantPromoterArabidopsis Ubi10 and MTAP1 (synthetic)Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-gRNA1-2(AtUBI10)-MTAP-LUC
Plasmid#234372PurposeT-DNA vector for expression of the two protein components of the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAsDepositorInsertsNbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-24XGP41
gRNA 1-1 and gRNA 1-2
UseSynthetic BiologyTagsGB1, GP41, and sfGFPExpressionPlantPromoterArabidopsis U6, Arabidopsis Ubi10, and MTAP1 (syn…Available SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTBL3342 PB-PEmax-puro
Plasmid#226674PurposePiggyBac cargo vector encoding PEmax and puromycin resistance.DepositorInsertPEmax
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
p35S-FHA-AtAGO4_gDNA
Plasmid#216838PurposeN-term Flag-HA tagged Arabidopsis thaliana (Col-0) AGO4 gDNA under regulation of CaMV 35S promoterDepositorAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 puro V3
Plasmid#226960PurposeCBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3346 PB-nicking guide-blast
Plasmid#226665PurposePiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidinDepositorInsertprotective nicking sgRNA
UseCRISPRExpressionMammalianPromoterhU6Available SinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2C
Plasmid#225070PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2C)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2A
Plasmid#225068PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2A (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2A)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2B
Plasmid#225069PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2B (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2B)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-57
Plasmid#225074PurposeExpresses two precursor peptides and the lanthipeptide synthetase from LanII-57 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanII-57)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-6
Plasmid#225076PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-6 (FAST-RiPPs) in E. coliDepositorInsertLanA(A) (LanIII-6)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-23
Plasmid#225067PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-23 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-23)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-109
Plasmid#218200PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-858
Plasmid#218008PurposemVenus fluorescent proteinDepositorInsertmVenus
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-853
Plasmid#218011PurposemCerulean fluorescent proteinDepositorInsertmCerulean
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-854
Plasmid#218012PurposemVenus fluorescent proteinDepositorInsertmVenus
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-855
Plasmid#218013PurposemCherry fluorescent proteinDepositorInsertmCherry
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-856
Plasmid#218014PurposesfGFP fluorescent proteinDepositorInsertsfGFP
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-872
Plasmid#218015PurposeNanoLuc luminescence reporterDepositorInsertNanoLuc
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-860
Plasmid#218016PurposemVenus fluorescent proteinDepositorInsertmVenus
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-861
Plasmid#218017PurposemCerulean fluorescent proteinDepositorInsertmCerulean
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-871
Plasmid#218019Purpose8xHis-1xHA-tag for protein detection and purificationDepositorInsert8xHis-1xHA-tag
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-1022
Plasmid#218020Purpose8xHis-SP20-3xHA-tag for protein detection and purificationDepositorInsert8xHis-SP20-1xHA-tag
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-869
Plasmid#218021PurposeER retention signal (DGDL) for protein targeting to ERDepositorInsertER retention signal (DGDL)
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-101
Plasmid#218199PurposeMulti-Stop for adding 3 stops for each frameDepositorInsertMulti-Stop
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-099
Plasmid#218201PurposeStrep-8xHis-tag for protein detection and purificationDepositorInsertStrep-8xHis
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-100
Plasmid#218202Purpose3xHA epitope tag for protein detectionDepositorInsert3xHA
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-723
Plasmid#218203Purpose1xHA-8xHis-tag for protein detection and purificationDepositorInsert1xHA-8xHis
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM0-054
Plasmid#218204PurposeSV40 NLS for protein targeting to nucleusDepositorInsertSV40 NLS
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-840
Plasmid#217996Purpose3xHA epitope tag for protein detectionDepositorInsert3xHA
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-843
Plasmid#217997PurposesmtHSP60 for protein targeting to mitochondrial matrixDepositorInsertsmtHSP60
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-844
Plasmid#217998PurposeNucleoplasmin NLS for protein targeting to nucleusDepositorInsertNucleoplasmin NLS
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-1125
Plasmid#217999PurposeStart-methionineDepositorInsertStart-Met
UseSynthetic BiologyAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBS-846
Plasmid#218001Purpose3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavageDepositorInsert3xFLAG-tag
UseSynthetic BiologyTagsPreScission protease cleavage siteAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only