We narrowed to 11,424 results for: 112

1. pX459-sgRNA3-RPB1-N-term

   Plasmid

   #195112

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against first exon of RPB1 (N-terminal)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pInducer10-UBC9-sh4

   Plasmid

   #168988

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDIRECT_21B

   Plasmid

   #91129

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-30a(+)-Syk-tSH2-PM

   Plasmid

   #111271

   Purpose

   express murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)

   Depositor

   Carol Post

   Article

   Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

   Insert

   murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y130E mutation to mimic phosphorylation

   Promoter

   T7 promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAD/CMV/V5-DEST-Rac1-2G

   Plasmid

   #66112

   Purpose

   Second generation Rac1 FRET biosensor for adenovirus production

   Depositor

   Olivier Pertz

   Article

   Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

   Insert

   Second generation Rac1 FRET biosensor
   

   Use

   Adenoviral

   Tags

   His-Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. vDip-C: pAAV-Pcp2-mGreenLantern-KASH

   Plasmid

   #207613

   Purpose

   vDip-C AAV vector with mouse Pcp2 (also known as L7) promoter driving green fluorophore mGreenLantern with a KASH nuclear membrane tag to isolate cell nuclei of cerebellar Purkinje cells

   Depositor

   Longzhi Tan

   Article

   Tan et al Science. 2023 Sep 8;381(6662):1112-1119.

   Insert

   mGreenLantern
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   mouse Pcp2 promoter (L7-6)

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS0035

   Plasmid

   #131125

   Purpose

   Mammalian Target-AID expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1 and UGI

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CMV promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Actin.CIB.GFP

   Plasmid

   #133618

   Purpose

   Expresses beta actin - CIBN - GFP protein fusion

   Depositor

   Robert Hughes

   Article

   Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

   Insert

   betaActin - CIB
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ActinS14V.CIB.GFP

   Plasmid

   #133620

   Purpose

   Expresses beta actin S14V - CIBN - GFP protein fusion

   Depositor

   Robert Hughes

   Article

   Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11

   Insert

   betaActin - CIB
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV-GSDMD_C192A-Myc

    Plasmid

    #201168

    Purpose

    Expression of GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    C192A

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AcrIIC1X

    Plasmid

    #128112

    Purpose

    AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

    Depositor

    Dominik Niopek

    Article

    Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

    Insert

    AcrX (AcrIIC1 N3F/D15Q/A48I)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA4/TO/myc-His-A-YAP2-deltaC

    Plasmid

    #21125

    Depositor

    Marius Sudol

    Article

    Tsutomu Oka & Marius et al

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 Carboxy-terminal amino acids -FLTWL are deleted

    Promoter

    Available since

    July 2, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Mito-Cyan Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71345

    Purpose

    Express Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    hOTC_Cyan Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. p35S::HY5

    Plasmid

    #135233

    Purpose

    constitutive expression of HY5 from A. thaliana (AT5G11260; downregulates UPR signaling)

    Depositor

    Armin Djamei

    Article

    Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

    Insert

    HY5 (HY5 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK597

    Plasmid

    #71121

    Purpose

    Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. KHBD00393

    Plasmid

    #34505

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6677 (ash2 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pFA6-CFAPoptim-KAN

    Plasmid

    #221128

    Purpose

    Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS413-TEF1pr-ANP1-FAPoptim

    Plasmid

    #221120

    Purpose

    FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    ANP1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge