We narrowed to 10,045 results for: Coli
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Plasmid#177231PurposeExpresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinaseDepositorInsertsgNeo1/sgNT1/sgNeo2
UseLentiviralPromoterbU6/mU6/hU6Available SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpec5'
Plasmid#228549PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpec3'
Plasmid#228548PurposeTemplate for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmidDepositorInsertTruncated 3' region of aadA
UseCRISPRAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_Cargo_T7_gutB
Plasmid#212139PurposeCargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.DepositorInsertxylitol dehydrogenase
ExpressionBacterialPromoterPT7Available SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCT03
Plasmid#212150PurposePlasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.DepositorInsertalanine dehydrogenase
Tags6x HisExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExp-TFSumo
Plasmid#112576PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-TFSumo and Strep tagExpressionBacterialAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-Tsf
Plasmid#112578PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Tsf (elongation factor Ts from E. coli fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis-Tsf and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHiKdtA
Plasmid#36269DepositorInsertH. influenzae KdtA
PromoteraraCAvailable SinceJune 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-WzxE-TEV-His10
Plasmid#226781PurposeExpression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coliDepositorInsertwzxE
TagsTEV cleavable 10xHistidine tagExpressionBacterialMutationvaline insertion in position 2 due to the cloning…PromoteraraBADAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW5126
Plasmid#129732Purposepolyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA proteinDepositorInserteatA-DUF::His8
Tags9His tagExpressionBacterialMutationreplaces the domain of unknown function correspon…PromoteraraBADAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-Ec1
Plasmid#138474PurposeEnables high-efficiency recombineering in E. coliDepositorInsertsCspRecT
mutL E32K
ExpressionBacterialMutationE32KAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KH-RBS2-dCas9
Plasmid#182740PurposeIntegrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoterDepositorInsertdCas9
UseCRISPR; Integrative vectorPromoterPtetAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only